荧光标记的凋亡诱导素在细胞摄取和反应研究中的应用。
The use of fluorescently-tagged apoptolidins in cellular uptake and response studies.
作者信息
Chong Katherine M, Leelatian Nalin, Deguire Sean M, Brockman Asa A, Earl David, Ihrie Rebecca A, Irish Jonathan M, Bachmann Brian O, Sulikowski Gary A
机构信息
Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.
出版信息
J Antibiot (Tokyo). 2016 Apr;69(4):327-30. doi: 10.1038/ja.2016.22. Epub 2016 Mar 9.
The apoptolidins are glycomacrolide microbial metabolites reported to be selectively cytotoxic against tumor cells. Using fluorescently tagged active derivatives we demonstrate selective uptake of these four tagged glycomacrolides in cancer cells over healthy human blood cells. We also demonstrate the utility of these five fluorescently tagged glycomacrolides in fluorescent flow cytometry to monitor cellular uptake of the six glycomacrolides and cellular response.
凋亡菌素是据报道对肿瘤细胞具有选择性细胞毒性的糖基大环内酯类微生物代谢产物。我们使用荧光标记的活性衍生物证明了这四种标记的糖基大环内酯类化合物在癌细胞中比健康人血细胞有选择性地摄取。我们还证明了这五种荧光标记的糖基大环内酯类化合物在荧光流式细胞术中用于监测六种糖基大环内酯类化合物的细胞摄取和细胞反应的效用。
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