State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Anal Chem. 2016 Apr 5;88(7):3864-71. doi: 10.1021/acs.analchem.6b00012. Epub 2016 Mar 18.
In this work, we present a novel energy-transfer (ET)-based photoelectrochemical (PEC) probing of DNA-protein interactions, which associates intimately with many important intracellular processes in transcriptional regulatory networks. Specifically, Au nanoparticles (NPs) were confined onto the CdS quantum dots (QDs) functionalized PEC surface by the formation of duplex DNA, the subsequent binding of the TATA binding protein (TBP) and the resulting distortion of the Au NPs capped DNA sequence could adjust the interparticle distance and thereby modulate the PEC performance of CdS QDs through the ET process between the CdS QDs and Au NPs. Using the duplex DNA sequence as a rigid spacer, the relationship between the photocurrent quenching effect and the spacing distance was also studied and some experimental conditions were optimized, on the basis of which a novel ET-based PEC TBP biosensor was realized with high sensitivity and selectivity.
在这项工作中，我们提出了一种基于能量转移（ET）的光电化学（PEC）探测 DNA-蛋白质相互作用的新方法，它与转录调控网络中许多重要的细胞内过程密切相关。具体来说，通过双链 DNA 的形成，将金纳米粒子（NPs）限制在 CdS 量子点（QDs）功能化的 PEC 表面上，随后 TATA 结合蛋白（TBP）的结合以及由此产生的 Au NPs 封端 DNA 序列的扭曲可以调整颗粒间的距离，从而通过 CdS QDs 和 Au NPs 之间的 ET 过程来调节 CdS QDs 的 PEC 性能。利用双链 DNA 序列作为刚性间隔物，还研究了光电流猝灭效应与间距之间的关系，并优化了一些实验条件，在此基础上，实现了一种具有高灵敏度和选择性的新型基于 ET 的 PEC TBP 生物传感器。
