乙酰芘标记的7-甲基鸟嘌呤核苷酸：异常的荧光特性及其在脱帽清除剂活性监测中的应用
Acetylpyrene-labelled 7-methylguanine nucleotides: unusual fluorescence properties and application to decapping scavenger activity monitoring.
作者信息
Kasprzyk Renata, Kowalska Joanna, Wieczorek Zbigniew, Szabelski Mariusz, Stolarski Ryszard, Jemielity Jacek
机构信息
Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, 02-089 Warsaw, Poland.
出版信息
Org Biomol Chem. 2016 Apr 28;14(16):3863-8. doi: 10.1039/c6ob00419a. Epub 2016 Mar 15.
7-Methylguanosine (m(7)G) nucleotides labelled with acetylpyrene (AcPy) were synthesized as fluorescent mRNA 5' end (cap) analogues. The unique fluorescent properties of m(7)G-AcPy conjugates, different from G-AcPy, can be applied to studying various mRNA cap-related processes including the evaluation of putative inhibitors of DcpS enzyme-a therapeutic target in neuromuscular diseases.
用乙酰芘（AcPy）标记的7-甲基鸟苷（m(7)G）核苷酸被合成为荧光mRNA 5'端（帽）类似物。m(7)G-AcPy缀合物独特的荧光特性不同于G-AcPy，可用于研究各种与mRNA帽相关的过程，包括评估DcpS酶的潜在抑制剂——一种神经肌肉疾病的治疗靶点。
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