丙环唑通过干扰侧链羟基化抑制油菜素内酯合成的体外和体内证据。
In vitro and in vivo evidence for the inhibition of brassinosteroid synthesis by propiconazole through interference with side chain hydroxylation.
作者信息
Oh Keimei, Matsumoto Tadashi, Hoshi Tomoki, Yoshizawa Yuko
机构信息
a Department of Biotechnology , Faculty of Bioresource Sciences, Akita Prefectural University , Shimoshinjo Nakano, Akita , Japan.
b National Agricultural Research Center, National Agriculture and Food Research Organization , Kannondai, Tsukuba , Ibaraki , Japan.
出版信息
Plant Signal Behav. 2016 May 3;11(5):e1158372. doi: 10.1080/15592324.2016.1158372.
We carried out the biochemical evaluation of the target site of propiconazole in BR biosynthesis. Applying BR biosynthesis intermediates to Arabidopsis seedlings grown in the presence of propiconazole under dark condition, we found that the target site of propiconazole in BR biosynthesis can be identified among the C22 and C23 side chain hydroxylation steps from campestanol to teasterone. Using differential spectra techniques to determine the binding affinity of propiconazole to CYP90D1, which is responsible for C23 hydroxylation of BR, we found that propiconazole induced typical type II binding spectra in response to purified recombinant CYP90D1 and the Kd value was found approximately 0.76 μM.
我们对丙环唑在油菜素内酯（BR）生物合成中的作用靶点进行了生化评估。在黑暗条件下，将BR生物合成中间体应用于在丙环唑存在下生长的拟南芥幼苗，我们发现，在从油菜甾烷醇到茶甾酮的C22和C23侧链羟基化步骤中，可以确定丙环唑在BR生物合成中的作用靶点。利用差示光谱技术测定丙环唑与负责BR C23羟基化的CYP90D1的结合亲和力，我们发现丙环唑对纯化的重组CYP90D1诱导出典型的II型结合光谱，并且发现解离常数（Kd）值约为0.76 μM。
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