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[用于构建代表性基因组文库的载体]

[Vectors for constructing representative genome libraries].

作者信息

Zabarovskiĭ E R, Allikmets R L

出版信息

Mol Biol (Mosk). 1989 Nov-Dec;23(6):1494-515.

PMID:2698992
Abstract

The general methodology for constructing genomic libraries of different types of vectors is discussed. Various ways of selection against non-recombinant molecules in generated libraries are considered. The general features of well-known vectors (lambda EMBL3, lambda EMBL4) and of new ones (lambda Ch40, lambda SK5, lambda FIX, pWE and other) are presented. A special attention is paid to vectors lambda SK9 and SK18 that have the features of lambda and M13 phages and of plasmids (diphasmids). Data on phasmids (lambda pMYF131 and lambda pSL51) and hyphages MC18 and MC19 are also presented.

摘要

讨论了构建不同类型载体基因组文库的一般方法。考虑了在生成的文库中针对非重组分子的各种选择方法。介绍了知名载体(λEMBL3、λEMBL4)和新载体(λCh40、λSK5、λFIX、pWE等)的一般特征。特别关注具有λ和M13噬菌体以及质粒(双相质粒)特征的载体λSK9和SK18。还介绍了有关噬菌粒(λpMYF131和λpSL51)以及丝状噬菌体MC18和MC19的数据。

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