Pini Núbia Inocencya Pavesi, Lima Débora Alves Nunes Leite, Sundfeld Renato Herman, Ambrosano Gláucia Maria Bovi, Aguiar Flavio Henrique Baggio, Lovadino José Roberto
Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil.
Department of Restorative Dentistry, Araçatuba Dental School, Araçatuba, São Paulo, Brazil.
J Investig Clin Dent. 2017 May;8(2). doi: 10.1111/jicd.12212. Epub 2016 Mar 20.
This study evaluated the effect of saliva on enamel after microabrasion with different microabrasive compounds under in situ conditions.
Enamel/dentin blocks (16 mm ) from bovine incisors were divided into nine groups (n = 19): one control group (no treatment), four groups treated with microabrasion using 35% phosphoric acid (H PO ) + pumice, and the last four groups treated with microabrasion using 6.6% hydrochloric acid (HCl) + silica. The treated groups were subdivided according to the in situ regimen: without salivary exposure, 1 h, 24 h, or 7 days of saliva exposure. Surface microhardness (SMH) and cross-sectional microhardness (CSMH) were tested. Scanning electron microscopy (SEM) was used to evaluate enamel morphology. Microhardness data were tested by analysis of variance, and Tukey's and Dunnett's tests (α = 0.05).
The SMH analysis revealed that all the microabrasion-treated groups presented significantly-reduced SMH values when compared to the control group (P < 0.05). Treatment with HCl + silica was more prone to the effect of saliva than H PO + pumice, even for CSMH analysis, once the superficial layers reached the same microhardness of that of the control group (P > 0.05). These results were confirmed by SEM, which demonstrated the mineral recovery effect over time.
Saliva was effective in promoting the rehardening of enamel after microabrasion, mainly for the surfaces treated with HCl + silica.
本研究评估了在原位条件下,不同微磨蚀剂微磨蚀后唾液对牙釉质的影响。
将牛切牙的牙釉质/牙本质块(16毫米)分为九组(n = 19):一组为对照组(未处理),四组使用35%磷酸(H₃PO₄)+浮石进行微磨蚀处理,最后四组使用6.6%盐酸(HCl)+二氧化硅进行微磨蚀处理。处理组根据原位方案进一步细分:无唾液暴露、唾液暴露1小时、24小时或7天。测试表面显微硬度(SMH)和横截面显微硬度(CSMH)。使用扫描电子显微镜(SEM)评估牙釉质形态。通过方差分析以及Tukey检验和Dunnett检验(α = 0.05)对显微硬度数据进行测试。
SMH分析显示,与对照组相比,所有微磨蚀处理组的SMH值均显著降低(P < 0.05)。即使对于CSMH分析,一旦表层达到与对照组相同的显微硬度(P > 0.05),HCl +二氧化硅处理比H₃PO₄ +浮石更容易受到唾液的影响。SEM证实了这些结果,其显示了随着时间推移矿物质的恢复效果。
唾液能有效促进微磨蚀后牙釉质的再硬化,主要针对用HCl +二氧化硅处理的表面。