Kubanov Alexey, Proshutinskaia Diana, Volnukhin Vladimir, Katunina Oksana, Abramova Tatiana
State Research Centre for Dermatovenereology and Cosmetology, Ministry of Health of the Russian Federation, Moscow, Russia.
Russian Medical Academy of Postgraduate Education Study, Moscow, Russia.
Acta Dermatovenerol Alp Pannonica Adriat. 2016;25(1):5-9. doi: 10.15570/actaapa.2016.2.
Occurence of vitiligo lesions is caused by the destruction of melanocytes in a ected skin and therefore by the re- duction of pigment melanin content. Questions remain about the presence of residual melanocytes in the depigmented skin and optimal methods of their identi cation.
Skin biopsy samples from 16 patients with non-segmental vitiligo and from 10 healthy volunteers were investigated for Melan-A (A103 clone)+ melanocytes expression by immunohistochemical analysis and for melanin by histochemical studies with section staining by Fontana-Masson method.
For some patients including those with long-standing disease (up to 40 years) Melan-A+ cells and melanin granules were detected in depigmented skin as indication that the residual melanocytes are preserved in vitiligo lesions. More than three-fold decrease of Melan-A+ melanocytes amount was revealed in perilesional normally pigmented skin of vitiligo patients (P < 0.001) compared with the skin of healthy volunteers. Clinically intact skin involvement in the pathological process should be taken into consideration if local treatment methods are prescribed.
In some vitiligo patients the residual melanocytes are preserved in depigmented skin. Melan-A marker is useful for identi cation of melanocytes in vitiligo patients' skin.
白癜风皮损的出现是由于受累皮肤中黑素细胞的破坏，进而导致色素黑素含量的减少。关于色素脱失皮肤中残余黑素细胞的存在情况及其最佳识别方法仍存在疑问。
通过免疫组织化学分析研究了16例非节段性白癜风患者和10名健康志愿者的皮肤活检样本中Melan-A（A103克隆）+黑素细胞的表达情况，并通过Fontana-Masson法切片染色的组织化学研究检测了黑素。
对于一些患者，包括病程长达40年的患者，在色素脱失皮肤中检测到Melan-A+细胞和黑素颗粒，这表明白癜风皮损中存在残余黑素细胞。与健康志愿者的皮肤相比，白癜风患者皮损周围正常色素沉着皮肤中Melan-A+黑素细胞数量减少了三倍以上（P<0.001）。如果采用局部治疗方法，应考虑临床上未受累皮肤参与病理过程的情况。
在一些白癜风患者中，色素脱失皮肤中存在残余黑素细胞。Melan-A标记物有助于识别白癜风患者皮肤中的黑素细胞。
