从盘藻（团藻目，绿藻门）中鉴定负交配型特异性基因MTD1(1)。
IDENTIFICATION OF THE MINUS MATING-TYPE SPECIFIC GENE MTD1 FROM GONIUM PECTORALE (VOLVOCALES, CHLOROPHYTA)(1).
作者信息
Hamaji Takashi, Ferris Patrick J, Nishii Ichiro, Nozaki Hisayoshi
机构信息
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, JapanPlant Biology Laboratory, Salk Institute, La Jolla, California 92037, USAAdvanced Science Institute, RIKEN, Wako-shi, Saitama 351-0198, JapanDepartment of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
出版信息
J Phycol. 2009 Dec;45(6):1310-4. doi: 10.1111/j.1529-8817.2009.00744.x. Epub 2009 Sep 29.
Gonium pectorale O. F. Müll. (Volvocales, Chlorophyta), a colonial 8- or 16-cellular alga, is phylogenetically important as an intermediate form between isogametic unicellular Chlamydomonas and oogamous Volvox. We identified the mating-type specific gene GpMTD1, from G. pectorale, the first homologue of Chlamydomonas reinhardtii MTD1 (CrMTD1). The GpMTD1 gene was found to be present only in the minus mating-type locus and was expressed specifically in the gametic phase as is the case for CrMTD1, suggested to participate in development of the minus gametes. This gene is useful as a probe in analyzing the bacterial artificial chromosome (BAC) library for resolving genomic structures of the mating-type loci in isogamous and oogamous colonial volvocaleans.
胸状团藻（O. F. Müll.）（绿藻门团藻目）是一种由8个或16个细胞组成的群体藻类，作为同配单细胞衣藻和卵配团藻之间的中间形式，在系统发育上具有重要意义。我们从胸状团藻中鉴定出了交配型特异性基因GpMTD1，它是莱茵衣藻MTD1（CrMTD1）的首个同源物。发现GpMTD1基因仅存在于负交配型位点，并且如CrMTD1一样在配子阶段特异性表达，提示其参与负配子的发育。该基因可作为探针，用于分析细菌人工染色体（BAC）文库，以解析同配和卵配群体团藻目植物交配型位点的基因组结构。
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