Xie Lihua, Yang Fangying, Sun Shenghua
Department of Respiratory Disease, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Department of Respiratory Disease, Fourth Hospital of Changsha, Changsha 410006, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Mar 28;41(3):238-43. doi: 10.11817/j.issn.1672-7347.2016.03.003.
To explore expression of miR-21 in peripheral blood serum and mononuclear cells of patients with chronic obstructive pulmonary disease (COPD), and to discuss the significance and underlying mechanisms.
The subjects were divided into a healthy control group (n=41) and a COPD group (n=49). The miR-21 level was detected by quantitative real-time PCR. The correlations between miR-21 and lung function or forced expiratory volume in one second (FEV1) were analyzed.
The expression levels of miR-21 in the serum and mononuclear cells in the COPD group were significantly elevated compared with those in the healthy group (P<0.05). The expression of miR-21 was correlated with the lung function of COPD patients. The expression level of miR-21 in the COPD patients was positively correlated with FEV1.
The upregulation of miR-21 in peripheral blood serum and mononuclear cells of COPD patients may contribute to the pathogenesis of COPD and the severity of this disease.
探讨微小RNA-21（miR-21）在慢性阻塞性肺疾病（COPD）患者外周血血清及单个核细胞中的表达情况，并探讨其意义及潜在机制。
将研究对象分为健康对照组（n = 41）和COPD组（n = 49）。采用定量实时聚合酶链反应检测miR-21水平。分析miR-21与肺功能或一秒用力呼气容积（FEV1）之间的相关性。
与健康组相比，COPD组血清及单个核细胞中miR-21的表达水平显著升高（P < 0.05）。miR-21的表达与COPD患者的肺功能相关。COPD患者中miR-21的表达水平与FEV1呈正相关。
COPD患者外周血血清及单个核细胞中miR-21的上调可能参与了COPD的发病机制及疾病严重程度。
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