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从新加坡周边沿海水域分离出的单细胞固氮蓝细菌(蓝藻)的形态学和系统发育研究(1)。

MORPHOLOGICAL AND PHYLOGENETIC STUDIES ON UNICELLULAR DIAZOTROPHIC CYANOBACTERIA (CYANOPHYTES) ISOLATED FROM THE COASTAL WATERS AROUND SINGAPORE(1).

作者信息

Ohki Kaori, Kamiya Mitsunobu, Honda Daisuke, Kumazawa Shuzo, Ho Kwok Ki

机构信息

The Faculty of Biotechnology, Fukui Prefectural University, Obama, Fukui 917-0003, JapanDepartment of Biology, Konan University, Kobe, Hyogo 658-8501, JapanSchool of Marine Science and Technology, Tokai University, Shimizu, Shizuoka 424-8610, JapanBiochemistry Department, Purdue University, West Lafayette, Indiana 47907-1153, USA.

出版信息

J Phycol. 2008 Feb;44(1):142-51. doi: 10.1111/j.1529-8817.2007.00428.x.

Abstract

Six unicellular diazotrophic cyanobacteria were isolated from the coast around Singapore. The isolates grew under both light:dark (L:D) cycles and continuous illumination (CL) in media without combined nitrogen and exhibited an ability to fix nitrogen (as measured by acetylene reduction) under aerobic conditions. The cells of all isolates were surrounded by a thick fibrous outer wall layer, and they divided by transverse binary fission. The arrangement of photosynthetic thylakoids was of the dispersed type. Three isolates were identified as form-genus Gloeothece as cells were divided in a single plane, and the other three isolates were identified as form-genus Gloeocapsa as cells were divided in multiple planes. Phylogenetic analyses based on the DNA sequences of the genes encoding 16S rRNA and dinitrogenase reductase (nifH) revealed the following: (i) Our six isolates formed a monophyletic cluster. (ii) The monophyletic cluster was subdivided into two phylogenetic groups, which taxonomically corresponded with the form-genera Gloeothece and Gloeocapsa. However, (iii) a diazotrophic strain of form-genus Gloeothece, Gloeothece membranacea (Rabenh.) Bornet PCC6501, was not closely related to our isolates, and (iv) some, but not all, diazotrophic unicellular strains of form-genus Cyanothece were observed to be in a close relationship with our isolates.

摘要

从新加坡周边海岸分离出了六种单细胞固氮蓝细菌。这些分离菌株在无化合态氮的培养基中,于光照:黑暗(L:D)循环和持续光照(CL)条件下均能生长,并且在有氧条件下表现出固氮能力(通过乙炔还原法测定)。所有分离菌株的细胞都被一层厚厚的纤维外壁层包围,它们通过横向二分分裂进行繁殖。光合类囊体的排列呈分散型。由于细胞在单一平面上分裂,三种分离菌株被鉴定为胶鞘藻属形态类;由于细胞在多个平面上分裂,另外三种分离菌株被鉴定为粘球藻属形态类。基于编码16S rRNA和固氮酶还原酶(nifH)的基因的DNA序列进行的系统发育分析表明:(i)我们的六种分离菌株形成了一个单系聚类。(ii)该单系聚类被细分为两个系统发育组,在分类学上分别对应于胶鞘藻属和粘球藻属形态类。然而,(iii)胶鞘藻属的一种固氮菌株,即膜状胶鞘藻(Rabenh.)Bornet PCC6501,与我们的分离菌株关系并不密切,并且(iv)观察到蓝囊藻属形态类的一些(但不是全部)固氮单细胞菌株与我们的分离菌株关系密切。

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