投喂不同水平的羽叶满江红对茶布罗鸡血液生化指标、血液学指标和免疫能力性状的影响。
Effect of feeding different levels of Azolla pinnata on blood biochemicals, hematology and immunocompetence traits of Chabro chicken.
作者信息
Mishra Deepesh Bharat, Roy Debashis, Kumar Vinod, Bhattacharyya Amitav, Kumar Muneendra, Kushwaha Raju, Vaswani Shalini
机构信息
Department of Animal Nutrition, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281001, Uttar Pradesh, India.
Department of Poultry Science, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281001, Uttar Pradesh, India.
出版信息
Vet World. 2016 Feb;9(2):192-8. doi: 10.14202/vetworld.2015.192-198. Epub 2016 Feb 20.
AIM
The present study was conducted to see the effect of feeding different levels of Azolla meal on blood biochemicals, hematology and immunocompetence traits of Chabro chicken.
MATERIALS AND METHODS
The study was conducted on 160 Chabro chicks, which were randomly divided into four treatment groups each with four replicates of 10 birds. The first treatment (T1) served as a control in which basal diets was offered without Azolla supplementation while in T2, T3, and T4 groups, basal diet was replaced with Azolla meal at 5%, 7.5%, and 10% levels, respectively. A feeding trial was conducted upto 8 weeks. At the last week of trial, blood samples were collected randomly from one bird of each replicate and plasma was separated to estimate certain biochemical parameters, some blood metabolites, minerals and enzymes like alanine aminotransferase and aspartate aminotransferase (AST). Hematological parameters such as hemoglobin, packed cell volume, total leukocytes count and differential leukocytes count were estimated in fresh blood just after collection. The humoral immune response was measured against sheep red blood cells,and cell-mediated immune response was measured against phyto hemagglutinin lectin from Phaseolus vulgaris (PHA-P).
RESULTS
The study showed that hematological profile of the Chabro bird was not affected by any treatment except heterophil and lymphocyte which was found higher in T2 and T3 groups and eosinophil was found higher in a T3 group than control. Blood glucose, creatinine, cholesterol, total protein, albumin, uric acid, and triglycerides were found similar in all the groups and within the normal values for broiler chicken. Liver enzymes and macro mineral content in blood were found similar in all the treatment groups and within normal physiological range. Although AST was found higher in 10% replacement group than control, the value was within normal range for broiler chicken. Although antibody titer was found similar in all the experimental groups in the present study, cell-mediate immune response (response to PHA-P) was found higher in 5%, 7.5%, and 10% replacement groups than control(p<0.05).
CONCLUSION
Similar blood biochemical parameters and higher cell-mediated immune response in Azolla replacement group indicated immune-modulatory effect of Azolla meal without any toxicity.
目的
本研究旨在观察饲喂不同水平满江红粉对查布罗鸡血液生化指标、血液学指标及免疫能力特征的影响。
材料与方法
本研究以160只查布罗雏鸡为对象，将其随机分为四个处理组，每组四个重复，每个重复10只鸡。第一个处理组（T1）作为对照组，饲喂基础日粮，不添加满江红；而在T2、T3和T4组中，基础日粮分别用5%、7.5%和10%水平的满江红粉替代。饲养试验持续8周。在试验的最后一周，从每个重复中随机选取一只鸡采集血样，分离血浆以估计某些生化参数、一些血液代谢物、矿物质以及丙氨酸氨基转移酶和天冬氨酸氨基转移酶（AST）等酶。采集新鲜血液后立即估计血红蛋白、红细胞压积、白细胞总数和白细胞分类计数等血液学参数。测定针对绵羊红细胞的体液免疫反应，以及针对菜豆的植物血凝素（PHA-P）的细胞介导免疫反应。
结果
研究表明，除嗜异性粒细胞和淋巴细胞外，查布罗鸡的血液学指标不受任何处理的影响，T2和T3组的嗜异性粒细胞和淋巴细胞高于对照组，T3组的嗜酸性粒细胞高于对照组。所有组的血糖、肌酐、胆固醇、总蛋白、白蛋白、尿酸和甘油三酯均相似，且在肉鸡的正常范围内。所有处理组血液中的肝酶和常量矿物质含量均相似，且在正常生理范围内。虽然10%替代组的AST高于对照组，但该值在肉鸡的正常范围内。虽然在本研究中所有实验组的抗体效价相似，但5%、7.5%和10%替代组的细胞介导免疫反应（对PHA-P的反应）高于对照组（p<0.05）。
结论
满江红替代组相似的血液生化参数和较高的细胞介导免疫反应表明满江红粉具有免疫调节作用且无任何毒性。
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