韩国发酵大豆酱豆瓣酱中产生毒素的蜡样芽孢杆菌和苏云金芽孢杆菌的存在情况。
Occurrence of Toxigenic Bacillus cereus and Bacillus thuringiensis in Doenjang, a Korean Fermented Soybean Paste.
作者信息
Park Kyung Min, Kim Hyun Jung, Jeong Moon Cheol, Koo Minseon
机构信息
Department of Food Biotechnology, Korea University of Science & Technology, Daejoen 305-333, South Korea, Department of Food Storage and Distribution System, Korea Food Research Institute, Gyeonggi 463-746, South Korea.
Department of Food Biotechnology, Korea University of Science & Technology, Daejoen 305-333, South Korea, Department of Food Safety, Korea Food Research Institute, Gyeonggi 463-746, South Korea.
出版信息
J Food Prot. 2016 Apr;79(4):605-12. doi: 10.4315/0362-028X.JFP-15-416.
This study determined the prevalence and toxin profile of Bacillus cereus and Bacillus thuringiensis in doenjang, a fermented soybean food, made using both traditional and commercial methods. The 51 doenjang samples tested were broadly contaminated with B. cereus; in contrast, only one sample was positive for B. thuringiensis. All B. cereus isolates from doenjang were positive for diarrheal toxin genes. The frequencies of nheABC and hblACD in traditional samples were 22.7 and 0%, respectively, whereas 5.1 and 5.1% of B. cereus isolates from commercial samples possessed nheABC and hblACD, respectively. The detection rate of ces gene was 10.8%. The predominant toxin profile among isolates from enterotoxigenic B. cereus in doenjang was profile 4 (entFM-bceT-cytK). The major enterotoxin genes in emetic B. cereus were cytK, entFM, and nheA genes. The B. thuringiensis isolate was of the diarrheagenic type. These results provide a better understanding of the epidemiology of the enterotoxigenic and emetic B. cereus groups in Korean fermented soybean products.
本研究测定了采用传统方法和商业方法制作的发酵大豆食品大酱中蜡样芽孢杆菌和苏云金芽孢杆菌的流行情况及毒素谱。所检测的51份大酱样品广泛受到蜡样芽孢杆菌污染；相比之下，只有一份样品对苏云金芽孢杆菌呈阳性。从大酱中分离出的所有蜡样芽孢杆菌菌株对腹泻毒素基因均呈阳性。传统样品中nheABC和hblACD的频率分别为22.7%和0%，而商业样品中蜡样芽孢杆菌分离株分别有5.1%和5.1%携带nheABC和hblACD。ces基因的检出率为10.8%。大酱中产肠毒素蜡样芽孢杆菌分离株中主要的毒素谱为谱型4（entFM-bceT-cytK）。致吐性蜡样芽孢杆菌中的主要肠毒素基因是cytK、entFM和nheA基因。苏云金芽孢杆菌分离株为腹泻型。这些结果有助于更好地了解韩国发酵大豆制品中产肠毒素和致吐性蜡样芽孢杆菌群的流行病学情况。
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