Deng Tao, Lu Liuliu, Gao Shipeng, Lin Ying, Han Shuangyan
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, Guangdong, China.
Biotechnol Lett. 2016 Jul;38(7):1139-45. doi: 10.1007/s10529-016-2088-1. Epub 2016 Apr 8.
To investigate the cellulose modification process on kraft pulp during recycling by mono-endoglucanase.
Pichia pastoris expressing endoglucanase, EG1, was grown in a 10 l fermenter yielding a high carboxymethyl cellulase (CMCase) activity of 340 U mg(-1). EG1-mediated modification of kraft pulp resulted in a paper sheet with the tensile index and burst index increased by 10 and 6.5 %, respectively. The kink index (indicating abrupt bends in fibres) of the enzyme-treated group decreased sharply by 45 % after the first recycling, compared with a reduction of only 1 % in the control group. Furthermore, EG1 treatment decreased the growth of crystallinity from 73.5 to 73.2 % and crystal size from 7.45 to 7.21 nm, which alleviated paper aging.
Endoglucanase EG1 modifies the interfacial properties of fibers, which affects fibre morphology during the recycling process and improves the technical properties of the resulting pulp and paper.
研究单内切葡聚糖酶在纸浆回收过程中对硫酸盐浆的纤维素改性过程。
表达内切葡聚糖酶EG1的毕赤酵母在10升发酵罐中培养，产生了340 U mg(-1)的高羧甲基纤维素酶（CMCase）活性。EG1介导的硫酸盐浆改性导致纸张的抗张指数和耐破指数分别提高了10%和6.5%。与对照组仅降低1%相比，酶处理组的扭结指数（表明纤维中的突然弯曲）在第一次回收后急剧下降了45%。此外，EG1处理使结晶度从73.5%降至73.2%，晶体尺寸从7.45纳米降至7.21纳米，从而缓解了纸张老化。
内切葡聚糖酶EG1改变了纤维的界面性质，这在回收过程中影响纤维形态，并改善了所得纸浆和纸张的技术性能。
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