Seifi-Skishahr Farnaz, Damirchi Arsalan, Farjaminezhad Manoochehr, Babaei Parvin
Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran; Department of Physical Education and Sport Sciences, Faculty of Education and Psychology, Mohaghegh Ardabili University, Ardabil, Iran.
Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran.
Biochem Res Int. 2016;2016:3757623. doi: 10.1155/2016/3757623. Epub 2016 Mar 15.
Objective. To assess the influence of different physical training status on exercise-induced oxidative stress and changes in cellular redox state. Methods. Thirty male subjects participated in this study and were assigned as well-trained (WT), moderately trained (MT), and untrained (UT) groups. The levels of cortisol, creatine kinase, plasma reduced glutathione to oxidized glutathione (GSH/GSSG), cysteine/cystine (Cys/CySS), and GSH/GSSG ratio in red blood cells (RBCs) were measured immediately and 10 and 30 min after exercise. Results. Following the exercise, plasma GSH/GSSG (p = 0.001) and Cys/CySS (p = 0.005) were significantly reduced in all groups. Reduction in plasma GSH/GSSG ratio in all groups induced a transient shift in redox balance towards a more oxidizing environment without difference between groups (p = 0.860), while RBCs GSH/GSSG showed significant reduction (p = 0.003) and elevation (p = 0.007) in UT and MT groups, respectively. The highest level of RBCs GSH/GSSG ratio was recorded in MT group, and the lowest one was recorded in the WT group. Conclusion. Long term regular exercise training with moderate intensity shifts redox balance towards more reducing environment, versus intensive exercise training leads to more oxidizing environment and consequently development of related diseases.
目的。评估不同体育锻炼状态对运动诱导的氧化应激及细胞氧化还原状态变化的影响。方法。30名男性受试者参与本研究，被分为训练良好组（WT）、中等训练组（MT）和未训练组（UT）。在运动后即刻、10分钟和30分钟测量皮质醇、肌酸激酶、血浆还原型谷胱甘肽与氧化型谷胱甘肽（GSH/GSSG）、半胱氨酸/胱氨酸（Cys/CySS）以及红细胞（RBC）中GSH/GSSG比值的水平。结果。运动后，所有组的血浆GSH/GSSG（p = 0.001）和Cys/CySS（p = 0.005）均显著降低。所有组血浆GSH/GSSG比值的降低导致氧化还原平衡短暂地向更具氧化性的环境转变，组间无差异（p = 0.860），而UT组和MT组的红细胞GSH/GSSG分别显著降低（p = 0.003）和升高（p = 0.007）。MT组的红细胞GSH/GSSG比值最高，WT组最低。结论。长期中等强度的规律运动训练使氧化还原平衡向更具还原性的环境转变，而高强度运动训练则导致更具氧化性的环境，进而引发相关疾病。
