Sotgia Salvatore, Arru Dionigia, Sotgiu Elisabetta, Mangoni Arduino A, Forteschi Mauro, Pintus Gianfranco, Carru Ciriaco, Zinellu Angelo
Department of Biomedical Sciences, School of Medicine, University of Sassari, Sassari, Italy.
Department of Clinical Pharmacology, School of Medicine, Flinders University, Adelaide, Australia.
Bioanalysis. 2016 May;8(9):945-51. doi: 10.4155/bio-2015-0002. Epub 2016 Apr 12.
Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC.
MATERIALS & METHODS: Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 µm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 µm) for CE analysis, monitoring the fluorescence of derivatives.
Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ.
This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.
建立了两种不同的巯基反应性碘乙酰试剂的柱前荧光衍生化方法，通过毛细管电泳（CE）和液相色谱（LC）同时测定人全血中的谷胱甘肽和L-麦角硫因。
采用反相柱（100 mm×4.6 mm Zorbax Eclipse Plus C18 3.5 µm）进行LC分析，在<5分钟内实现分离；采用未涂层熔融石英毛细管（60 cm×50 µm）进行CE分析，监测衍生物的荧光。
该测定方法在线性、回收率、日内和日间精密度以及检测限和定量限方面表现良好。
这种新方法能够快速评估循环中的谷胱甘肽和L-麦角硫因浓度，用于临床和研究目的。
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