[Determination of anticonvulsive preparations in the blood serum of patients by high-performance liquid chromatography].

A method for simultaneous measurement of hexamydine, phenobarbital, diphenin and carbamazepine concentrations in serum using high performance liquid chromatography is described. The procedure employs acetonitrile extraction of anticonvulsants and chromatography of the extract under the following conditions: a column containing lichrosorb RP-8 10 microns (250 x 4 mm), mobile phase consisting of 30 vol.% ethanol solution in 0.01 M KH2PO4 pH 5.3, flow rate 0.5 ml/min, column temperature 30 degrees C, injection volume 20 microliters, detector wavelength 220 nm. Hexenal is used as an internal standard. The concentration range is 5-50 mg/l for phenobarbital, diphenin and carbamazepine and 5-30 mg/l for hexamydine. The relative standard deviation varies between 0.018 and 0.057 for therapeutic and toxic concentrations.