Pillar of Engineering Product Development, Singapore University of Technology and Design , Singapore 487372, Singapore.
Anal Chem. 2016 May 17;88(10):5316-23. doi: 10.1021/acs.analchem.6b00605. Epub 2016 Apr 27.
Components in biomedical analysis tools that have direct contact with biological samples, especially biohazardous materials, are ideally discarded after use to prevent cross-contamination. However, a conventional acoustofluidic device is typically a monolithic integration that permanently bonds acoustic transducers with microfluidic channels, increasing processing costs in single-use platforms. In this study, we demonstrate a detachable acoustofluidic system comprised of a disposable channel device and a reusable acoustic transducer for noncontact continuous particle separation via a traveling surface acoustic wave (TSAW). The channel device can be placed onto the SAW transducer with a high alignment tolerance to simplify operation, is made entirely of polydimethylsiloxane (PDMS), and does not require any additional coupling agent. A microstructured pillar is used to couple acoustic waves into the fluid channel for noncontact particle manipulation. We demonstrate the separation of 10 and 15 μm particles at high separation efficiency above 98% in a 49.5 MHz TSAW using the developed detachable acoustofluidic system. Its disposability and ease of assembly should enable broad use of noncontact, disposable particle manipulation techniques in practical biomedical applications related to sample preparation.
在生物医学分析工具中，与生物样本（尤其是生物危害性材料）直接接触的部件在使用后理想情况下应丢弃，以防止交叉污染。然而，传统的声流控装置通常是一种整体式集成，将声学换能器与微流道永久性地结合在一起，这增加了一次性平台的处理成本。在本研究中，我们展示了一种由可抛弃式通道器件和可重复使用的声学换能器组成的可拆卸声流系统，用于通过行波表面声波（TSAW）实现非接触连续粒子分离。该通道器件可通过高对准容差放置在 SAW 换能器上，简化操作，完全由聚二甲基硅氧烷（PDMS）制成，并且不需要任何额外的耦合剂。微结构柱用于将声波耦合到流体通道中，以进行非接触式粒子操纵。我们展示了使用所开发的可拆卸声流系统，在 49.5MHz 的 TSAW 中以高于 98%的高分离效率分离 10 和 15μm 的粒子。其一次性使用和易于组装的特点，应该能够在与样品制备相关的实际生物医学应用中广泛应用非接触式、一次性粒子操纵技术。
