Dugat T, Haciane D, Durand B, Lagrée A-C, Haddad N, Boulouis H-J
Laboratoire de Santé Animale, UMR BIPAR, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail, Université Paris-Est, Maisons-Alfort, France.
UMR BIPAR, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, France.
Transbound Emerg Dis. 2017 Oct;64(5):e1-e3. doi: 10.1111/tbed.12508. Epub 2016 Apr 19.
Anaplasma phagocytophilum is a tick-borne pathogen that causes tick-borne fever in domestic ruminants. Tick-borne fever is characterized by diverse symptoms and occasionally causes abortions in domestic ruminants, resulting in significant economic impact. However, in France, the potential frequency of A. phagocytophilum-related abortions is unknown, and thus, it remains difficult to estimate the full extent of its economic impact. This gap in our knowledge is likely explained, at least in part, by the absence of suitable and specific tools capable of detecting A. phagocytophilum associated with abortion. Our objective was to identify a genetic marker able to differentiate A. phagocytophilum strains isolated from domestic ruminants that had aborted compared to those that had not. Thus, we typed a total of 123 A. phagocytophilum obtained from cattle, of which 25 originated from cows that had aborted, via multiple-locus variable-number tandem repeat (VNTR) analysis. These included 56 new A. phagocytophilum samples and 67 previously published A. phagocytophilum samples. A multivariate logistic model demonstrated that the triple-repeat allele of the APV-A VNTR was statistically more frequent in A. phagocytophilum from cattle that had aborted, compared to A. phagocytophilum from cattle that had not aborted (P = 0.03), while controlling for any regional effects (P < 0.0001). For four other VNTR, there were no statistical associations between specific alleles and abortion. The APV-A triple-repeat VNTR allele could thus act as a marker of A. phagocytophilum involved in abortions. If this hypothesis is confirmed in additional samples from other regions, this marker could then be utilized in the development of A. phagocytophilum abortive strain surveillance measures.
嗜吞噬细胞无形体是一种蜱传病原体，可导致家养反刍动物患蜱传热。蜱传热症状多样，偶尔会导致家养反刍动物流产，造成重大经济影响。然而，在法国，与嗜吞噬细胞无形体相关的流产潜在发生率尚不清楚，因此，仍然难以估计其经济影响的全部程度。我们知识上的这一差距可能至少部分是由于缺乏能够检测与流产相关的嗜吞噬细胞无形体的合适且特异的工具。我们的目标是鉴定一种遗传标记，能够区分从流产的家养反刍动物分离出的嗜吞噬细胞无形体菌株与未流产的菌株。因此，我们通过多位点可变数目串联重复序列（VNTR）分析，对总共123株从牛身上获得的嗜吞噬细胞无形体进行了分型，其中25株来自流产的母牛。这些包括56个新的嗜吞噬细胞无形体样本和67个先前发表的嗜吞噬细胞无形体样本。多变量逻辑模型表明，与未流产牛的嗜吞噬细胞无形体相比，APV-A VNTR的三重复等位基因在流产牛的嗜吞噬细胞无形体中在统计学上更为常见（P = 0.03），同时控制了任何区域效应（P < 0.0001）。对于其他四个VNTR，特定等位基因与流产之间没有统计学关联。因此，APV-A三重复VNTR等位基因可作为与流产相关的嗜吞噬细胞无形体的标记。如果这一假设在来自其他地区的更多样本中得到证实，那么该标记可用于制定嗜吞噬细胞无形体流产菌株监测措施。
