Agüera Eduardo, Castilla Salvador, Luque Evelio, Jimena Ignacio, Leiva-Cepas Fernando, Ruz-Caracuel Ignacio, Peña José
Department of Neurology, Reina Sofia University Hospital, Córdoba, Spain.
Maimonides Institute for Biomedical Research IMIBIC, Reina Sofia University Hospital, University of Cordoba, Córdoba, Spain.
Histol Histopathol. 2016 Dec;31(12):1367-79. doi: 10.14670/HH-11-776. Epub 2016 Apr 19.
This study was conducted to determine the effects of extracts obtained from both normal and denervated muscles on different muscle types. Wistar rats were used and were divided into a control group and four experimental groups. Each experimental group was treated intraperitoneally during 10 consecutive days with a different extract. These extracts were obtained from normal soleus muscle, denervated soleus, normal extensor digitorum longus, and denervated extensor digitorum longus. Following treatment, the soleus and extensor digitorum longus muscles were obtained for study under optic and transmission electron microscope; morphometric parameters and myogenic responses were also analyzed. The results demonstrated that the treatment with normal soleus muscle and denervated soleus muscle extracts provoked hypertrophy and increased myogenic activity. In contrast, treatment with extracts from the normal and denervated EDL had a different effect depending on the muscle analyzed. In the soleus muscle it provoked hypertrophy of type I fibers and increased myogenic activity, while in the extensor digitorum longus atrophy of the type II fibers was observed without changes in myogenic activity. This suggests that the muscular responses of atrophy and hypertrophy may depend on different factors related to the muscle type which could be related to innervation.
本研究旨在确定从正常肌肉和失神经肌肉中提取的提取物对不同肌肉类型的影响。使用了Wistar大鼠，并将其分为一个对照组和四个实验组。每个实验组连续10天腹腔注射不同的提取物。这些提取物分别来自正常比目鱼肌、失神经比目鱼肌、正常趾长伸肌和失神经趾长伸肌。治疗后，获取比目鱼肌和趾长伸肌用于光学显微镜和透射电子显微镜下研究；还分析了形态计量学参数和肌源性反应。结果表明，用正常比目鱼肌和失神经比目鱼肌提取物治疗可引起肥大并增加肌源性活性。相比之下，用正常和失神经趾长伸肌提取物治疗根据所分析的肌肉产生不同的效果。在比目鱼肌中，它引起I型纤维肥大并增加肌源性活性，而在趾长伸肌中观察到II型纤维萎缩，肌源性活性无变化。这表明萎缩和肥大的肌肉反应可能取决于与肌肉类型相关的不同因素，这些因素可能与神经支配有关。
