Yang Cuiyun, Fang Shengtao, Chen Dehui, Wang Jianhua, Liu Fanghua, Xia Chuanhai
Key Laboratory of Coastal Biology and Biological Research Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
Department of Biology, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China.
Mar Pollut Bull. 2016 Jun 15;107(1):118-124. doi: 10.1016/j.marpolbul.2016.04.010. Epub 2016 Apr 16.
Bacterial quorum sensing signal molecules N-acyl homoserine lactones (AHLs) (C10-HSL, 3-OXO-C10-HSL and 3-OH-C10-HSL) as possible chemical cues were employed to investigate the role in the formation of fouling diatom-biofilm (Cylindrotheca sp.). Results showed that AHLs promoted Chlorophyll a (Chl.a) and extracellular polymeric substance (EPS) contents in the diatom-biofilm. In the presence of AHLs-inhibitor 3, 4-Dibromo-2(5)H-furanone, which was used to avoid the possible interference of AHLs from bacteria, AHLs also increased the Chl.a and EPS contents. Scanning electron microscope and confocal laser scanning microscope analysis further demonstrated that AHLs promoted the formation of the diatom-biofilm. Non-invasive micro-test technique showed that AHLs promoted Ca(2+) efflux in Cylindrotheca sp., which implied that Ca(2+) might be correlated with AHLs-induced positive effect on the formation of diatom-biofilm. This study provides direct evidences that AHLs play an important role in developing the diatom-biofilm and AHLs-inhibitors might be promising active agents in marine antifouling.
细菌群体感应信号分子N-酰基高丝氨酸内酯（AHLs）（C10-HSL、3-氧代-C10-HSL和3-羟基-C10-HSL）作为可能的化学信号被用于研究其在污损硅藻生物膜（圆柱鞘藻属）形成中的作用。结果表明，AHLs促进了硅藻生物膜中叶绿素a（Chl.a）和胞外聚合物（EPS）的含量。在使用AHLs抑制剂3,4-二溴-2(5)H-呋喃酮以避免细菌产生的AHLs可能产生的干扰的情况下，AHLs仍增加了Chl.a和EPS的含量。扫描电子显微镜和共聚焦激光扫描显微镜分析进一步证明，AHLs促进了硅藻生物膜的形成。非损伤微测试技术表明，AHLs促进了圆柱鞘藻属中Ca(2+)的外流，这意味着Ca(2+)可能与AHLs对硅藻生物膜形成的诱导积极作用相关。本研究提供了直接证据，表明AHLs在硅藻生物膜形成中起重要作用，且AHLs抑制剂可能是有前景的海洋防污活性剂。
