Wang Weiyun, Liu Wanyu, Wang Lingyun, Yang Tianhua, Li Rundong
J Environ Biol. 2016 Mar;37(2):305-12.
Extracellular polymer substance (EPS) is an important substance in sludge dewatering process and can be divided into slime, loosely bound EPS(LB-EPS) and tightly bound EPS(TB-EPS). The major components in different EPS layers in mechanical dewatered sludge (sample D) and sludge from thickening tank (sample U) were analyzed in the study. Results showed that, for all sludge samples, protein content in slime was much higher than that in LB-EPS and TB-EPS. In addition, from the results of total EPS content in four sludge samples (U: 98.68 mg gvss⁻¹, US (sample U+SDS): 135.27 mg gvss⁻¹, D:138.16 mg gvss⁻¹, DS:211.21 mg gvss⁻¹), it was found that sodium dodecyl sulfate (SDS) extracted more EPS content from sludge, which proved that SDS might cause substantial release of EPS, especially PN from sludge. Increase PS content in LB-EPS, and TB-EPS as addition of SDS, implied sensibility of cells in sludge to SDS. Differential Scanning Calorimetry found that bound water proportion in four different sludge samples ranged as follows: as D>DS>US>U. Results from other analytical techniques,such as FTIR and NMR, were also given the supported proof for the distribution changes of EPS and bound water.
胞外聚合物（EPS）是污泥脱水过程中的一种重要物质，可分为黏液、松散结合型EPS（LB-EPS）和紧密结合型EPS（TB-EPS）。本研究分析了机械脱水污泥（样品D）和浓缩池污泥（样品U）中不同EPS层的主要成分。结果表明，对于所有污泥样品，黏液中的蛋白质含量远高于LB-EPS和TB-EPS中的蛋白质含量。此外，从四个污泥样品的总EPS含量结果（U：98.68 mg gvss⁻¹，US（样品U+SDS）：135.27 mg gvss⁻¹，D：138.16 mg gvss⁻¹，DS：211.21 mg gvss⁻¹）发现，十二烷基硫酸钠（SDS）从污泥中提取了更多的EPS含量，这证明SDS可能导致EPS大量释放，尤其是污泥中的PN。随着SDS的添加，LB-EPS和TB-EPS中的PS含量增加，这意味着污泥中的细胞对SDS敏感。差示扫描量热法发现，四种不同污泥样品中的结合水比例如下：D>DS>US>U。傅里叶变换红外光谱（FTIR）和核磁共振（NMR）等其他分析技术的结果也为EPS和结合水的分布变化提供了支持证据。
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