Sánchez-Chapul Laura, Ángel-Muñoz Miguel Del, Ruano-Calderón Luis, Luna-Angulo Alexandra, Coral-Vázquez Ramón, Hernández-Hernández Óscar, Magaña Jonathan J, León-Hernández Saúl R, Escobar-Cedillo Rosa E, Vargas Steven
División de Neurociencias, Instituto Nacional de Rehabilitación (INR), Calz. Mexico-Xochimilco 289, Col. Arenal de Guadalupe, Del. Tlalpan, 14389, Mexico City, Mexico.
Subdirección de Enseñanza y Capacitación, Investigación y Calidad en Salud, Secretaría de Salud del Estado de Durango (SSED), Durango City, Mexico.
Muscle Nerve. 2016 Dec;54(6):1064-1071. doi: 10.1002/mus.25156. Epub 2016 Oct 11.
In this study, we determined normal levels of dysferlin expression in CD14 monocytes by flow cytometry (FC) as a screening tool for dysferlinopathies.
Monocytes from 183 healthy individuals and 29 patients were immunolabeled, run on an FACScalibur flow cytometer, and analyzed by FlowJo software.
The relative quantity of dysferlin was expressed as mean fluorescence intensity (MFI). Performance of this diagnostic test was assessed by calculating likelihood ratios at different MFI cut-off points, which allowed definition of 4 disease classification groups in a simplified algorithm.
The MFI value may differentiate patients with dysferlinopathy from healthy individuals; it may be a useful marker for screening purposes. Muscle Nerve 54: 1064-1071, 2016.
在本研究中，我们通过流式细胞术（FC）测定了CD14单核细胞中dysferlin的正常表达水平，作为肢带型肌营养不良症的筛查工具。
对183名健康个体和29名患者的单核细胞进行免疫标记，在FACScalibur流式细胞仪上检测，并通过FlowJo软件进行分析。
dysferlin的相对量以平均荧光强度（MFI）表示。通过计算不同MFI切点的似然比来评估该诊断测试的性能，这使得可以在简化算法中定义4个疾病分类组。
MFI值可区分肢带型肌营养不良症患者与健康个体；它可能是用于筛查目的的有用标志物。《肌肉与神经》54: 1064 - 1071, 2016年。
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