Trocan Ilie, Ceausu Raluca A, Jitariu Andreea A, Haragus Horia, Damian Gratian, Raica Marius
Department of Orthopedics and Trauma, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
Department of Microscopic Morphology/Histology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
In Vivo. 2016 May-Jun;30(3):225-30.
The aim of this study was to analyze the microstructural architecture and cellular differentiation of the anterior cruciate ligament (ACL) stumps in different stages after injury, as this could augment graft biointegration.
The histological appearance and immunoreaction for cluster of differentiation 34 antigen (CD34) of 54 biopsies from 27 remnants were compared to 10 biopsies from 5 normal cruciate ligaments.
CD34 reaction in endothelial cells, fibroblasts and fibrocytes was consistently positive in small synovial vessels. Remnants also exhibited CD34(+) cells among collagen fibers. Blood vessel density varied between specimens. The mean vascular microdensity was 43 per ×200 field in remnants compared to 15.2 in controls. A total of 94.44% of remnant ACL samples had significant hyperplasia of stellate and fusiform stromal cells, CD34(+); 22.4% had developed capillary vessels inside the ligament; 33% exhibited ongoing angiogenesis.
Significant differences exist between torn and intact ACL regarding microvascularization. The remnants contain stellate stromal cells and CD34(+) fibrocytes, and display angiogenesis both at synovia as well as in the ligament itself. These findings underline the potential contribution to neoligament healing when remnants are preserved.
本研究旨在分析前交叉韧带（ACL）损伤后不同阶段残端的微观结构和细胞分化情况，因为这可能会增强移植物的生物整合。
将来自27个残端的54份活检标本的组织学表现和分化簇34抗原（CD34）免疫反应与来自5条正常交叉韧带的10份活检标本进行比较。
内皮细胞、成纤维细胞和纤维细胞中的CD34反应在小滑膜血管中始终呈阳性。残端在胶原纤维中也表现出CD34（+）细胞。不同标本间血管密度不同。残端中每200视野的平均血管微密度为43，而对照组为15.2。总共94.44%的ACL残端样本有星状和梭形基质细胞（CD34（+））显著增生；22.4%在韧带内部形成了毛细血管；33%表现出正在进行的血管生成。
撕裂的和完整的ACL在微血管化方面存在显著差异。残端含有星状基质细胞和CD34（+）纤维细胞，并且在滑膜以及韧带本身都表现出血管生成。这些发现强调了保留残端对新韧带愈合的潜在贡献。
Zotero 插件