从有医院感染高风险的儿科患者中分离出的耐药肠球菌毒力基因检测。
Detection of virulence genes in resistant enterococci isolated from pediatric patients at high risk for nosocomial infections.
作者信息
Nateghian Alireza, Fallah Fatemeh, Daghighi Zahra, Goudarzi Hossein, Hashemi Ali, Robinson Joan L
机构信息
Iran University of Medical Sciences, Ali-Asghar Children's Hospital, Tehran, Iran.
Pediatric Infections Research Center, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Diagn Microbiol Infect Dis. 2016 Jun;85(2):260-2. doi: 10.1016/j.diagmicrobio.2016.03.020. Epub 2016 Mar 31.
BACKGROUND
The study of virulence genes carried by enterococci has become of greater relevance as nosocomial enterococcal infections have become more prevalent and possibly more severe.
METHODS
Surveillance swabs were performed on children less than 18 months of age in an intensive care unit in Iran in 2012-2013. Multiplex PCR and sequencing methods were used to detect gelE, esp and asa1 genes in enterococci with intermediate or full resistance to vancomycin.
RESULTS
The rate of carriage of the genes was gelE (91%), esp (79%) and asa1 (87%).
CONCLUSION
The majority of enterococcal strains with resistance to vancomycin carry genes for all three potential virulence factors that were analyzed in this study. This might explain why enterococcal infections appear to be more virulent than in the past.
背景
随着医院内肠球菌感染变得更加普遍且可能更严重，对肠球菌携带的毒力基因的研究变得愈发重要。
方法
2012 - 2013年在伊朗一家重症监护病房对18个月以下儿童进行监测拭子采样。采用多重PCR和测序方法检测对万古霉素呈中度或完全耐药的肠球菌中的gelE、esp和asa1基因。
结果
这些基因的携带率分别为gelE（91%）、esp（79%）和asa1（87%）。
结论
大多数对万古霉素耐药的肠球菌菌株携带本研究中分析的所有三种潜在毒力因子的基因。这可能解释了为什么肠球菌感染似乎比过去更具毒性。
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