Suppr超能文献

石榴叶提取物通过线粒体内在途径诱导非小细胞肺癌细胞凋亡,并抑制其体外迁移和侵袭。

Punica granatum (pomegranate) leaves extract induces apoptosis through mitochondrial intrinsic pathway and inhibits migration and invasion in non-small cell lung cancer in vitro.

机构信息

Department of Nutrition and Food Hygiene, School of Public Health, West China Medical School, Sichuan University, 17# 3rd Section, Ren Min South Road, 610041 Chengdu, China.

State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, 17# 3rd Section, Ren Min South Road, 610041 Chengdu, China.

出版信息

Biomed Pharmacother. 2016 May;80:227-235. doi: 10.1016/j.biopha.2016.03.023. Epub 2016 Mar 28.

Abstract

Most conventional treatments on non-small cell lung carcinoma always accompany with awful side effects, and the incidence and mortality rates of this cancer are increasing rapidly worldwide. The objective of this study was to examine the anticancer effects of extract of Punica granatum (pomegranate) leaves extract (PLE) on the non-small cell lung carcinoma cell line A549, H1299 and mouse Lewis lung carcinoma cell line LL/2 in vitro, and explore its mechanisms of action. Our results have shown that PLE inhibited cell proliferation in non-small cell lung carcinoma cell line in a concentration- and time-dependent manner. Flow cytometry (FCM) assay showed that PLE affected H1299 cell survival by arresting cell cycle progression in G2/M phase in a dose-dependent manner and inducing apoptosis. Moreover, PLE could also decrease the reactive oxygen species (ROS) and the mitochondrial membrane potential (ΔYm), indicating that PLE may induce apoptosis via mitochondria-mediated apoptotic pathway. Furthermore, PLE blocked H1299 cell migration and invasion, and the reduction of matrix metalloproteinase (MMP) MMP-2 and MMP-9 expression were also observed in vitro. These results suggested that PLE could be an effective and safe chemotherapeutic agent in non-small cell lung carcinoma treatment by inhibiting proliferation, inducing apoptosis, cell cycle arrest and impairing cell migration and invasion.

摘要

大多数非小细胞肺癌的常规治疗方法往往伴随着严重的副作用,而且这种癌症的发病率和死亡率在全球范围内迅速上升。本研究旨在探讨石榴叶提取物(PLE)对非小细胞肺癌细胞系 A549、H1299 和小鼠 Lewis 肺癌细胞系 LL/2 的体外抗癌作用,并探讨其作用机制。我们的研究结果表明,PLE 呈浓度和时间依赖性地抑制非小细胞肺癌细胞系的增殖。流式细胞术(FCM)检测表明,PLE 通过诱导细胞周期 G2/M 期阻滞和凋亡,以剂量依赖性方式影响 H1299 细胞的存活。此外,PLE 还可以降低活性氧(ROS)和线粒体膜电位(ΔYm),表明 PLE 可能通过线粒体介导的凋亡途径诱导细胞凋亡。此外,PLE 还能阻断 H1299 细胞的迁移和侵袭,并且在体外观察到基质金属蛋白酶(MMP)MMP-2 和 MMP-9 的表达减少。这些结果表明,PLE 可能通过抑制增殖、诱导凋亡、细胞周期阻滞以及损害细胞迁移和侵袭,成为一种有效和安全的非小细胞肺癌治疗化疗药物。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验