Asmar M, Arngrim N, Simonsen L, Asmar A, Nordby P, Holst J J, Bülow J
Department of Clinical Physiology/Nuclear Medicine, Bispebjerg University Hospital, Copenhagen, Denmark.
NNF Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.
Nutr Diabetes. 2016 May 2;6(5):e208. doi: 10.1038/nutd.2016.15.
Glucose-dependent insulinotropic polypeptide (GIP) appears to have impaired effect on subcutaneous abdominal adipose tissue metabolism in obese subjects. The aim of the present study was to examine whether weight loss may reverse the impaired effect of GIP on subcutaneous abdominal adipose tissue in obese subjects.
Five obese males participated in a 12-week weight loss program, which consisted of caloric restriction (800 Cal day(-)(1)) followed by 4 weeks of weight-maintenance diet. Before and after weight loss, subcutaneous adipose tissue lipid metabolism was studied by conducting regional measurements of arterio-venous plasma concentrations of metabolites and blood flow (adipose tissue blood flow, ATBF) across a segment of the abdominal adipose tissue in the fasting state and during GIP infusion (1.5 pmol kg(-)(1 )min(-)(1)) in combination with a hyperinsulinemic-hyperglycemic clamp.
After weight loss (7.5±0.8 kg), glucose tolerance and insulin sensitivity increased significantly as expected. No significant differences were seen in basal ATBF before (1.3±0.4 ml min(-1) 100 g tissue(-1)) and after weight loss (2.1±0.4 ml min(-1) 100 g tissue)(-1); however, a tendency to increase was seen. After weight loss, GIP infusion increased ATBF significantly (3.2±0.1 ml min(-1) 100 g tissue(-1)) whereas there was no increase before weight loss. Triacylglycerol (TAG) uptake did not change after weight loss. Baseline free fatty acid (FFA) and glycerol output increased significantly after weight loss, P<0.001. During the clamp period, FFA and glycerol output declined significantly, P<0.05, with no differences before and after weight loss. Weight loss increased glucose uptake and decreased FFA/glycerol ratio during the clamp period, P<0.05.
In obese subjects, weight loss, induced by calorie restriction, improves the blunted effect of GIP on subcutaneous abdominal adipose tissue metabolism.
在肥胖受试者中，葡萄糖依赖性促胰岛素多肽（GIP）对腹部皮下脂肪组织代谢的作用似乎受损。本研究旨在探讨体重减轻是否可逆转肥胖受试者中GIP对腹部皮下脂肪组织的受损作用。
五名肥胖男性参与了一项为期12周的体重减轻计划，该计划包括热量限制（800千卡/天），随后是4周的体重维持饮食。在体重减轻前后，通过在空腹状态下以及在GIP输注（1.5皮摩尔/千克/分钟）期间，结合高胰岛素-高血糖钳夹技术，对腹部脂肪组织一段区域的动静脉血浆代谢物浓度和血流量（脂肪组织血流量，ATBF）进行区域测量，研究皮下脂肪组织脂质代谢。
体重减轻（7.5±0.8千克）后，正如预期的那样，葡萄糖耐量和胰岛素敏感性显著增加。体重减轻前（1.3±0.4毫升/分钟/100克组织）和体重减轻后（2.1±0.4毫升/分钟/100克组织）的基础ATBF无显著差异；然而，有增加的趋势。体重减轻后，GIP输注显著增加了ATBF（3.2±0.1毫升/分钟/100克组织），而体重减轻前没有增加。体重减轻后三酰甘油（TAG）摄取没有变化。体重减轻后，基线游离脂肪酸（FFA）和甘油输出显著增加，P<0.001。在钳夹期，FFA和甘油输出显著下降，P<0.05，体重减轻前后无差异。体重减轻增加了钳夹期的葡萄糖摄取并降低了FFA/甘油比值，P<0.05。
在肥胖受试者中，热量限制引起的体重减轻改善了GIP对腹部皮下脂肪组织代谢的钝化作用。
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