从小鼠中分离树突状细胞祖细胞和骨髓祖细胞。

Isolation of Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse.

作者信息

Onai Nobuyuki, Ohteki Toshiaki

机构信息

Department of Biodefense, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo, 113-8510, Japan.

出版信息

Methods Mol Biol. 2016;1423:53-9. doi: 10.1007/978-1-4939-3606-9_4.

DOI:10.1007/978-1-4939-3606-9_4

PMID:27142008

Abstract

Dendritic cells (DCs) comprise two major subsets, conventional DC (cDC) and plasmacytoid DC (pDC) in the steady-state lymphoid organ. These cells have a short half-life and therefore, require continuous generation from hematopoietic stem cells and progenitor cells. Recently, we identified DC-restricted progenitors called common DC progenitors (CDPs) in the bone marrow of mouse. The CDPs can be isolated from mouse bone marrow based on the hematopoietic cytokine receptors, such as Flt3 (Fms-related tyrosine kinase 3) (CD135), c-kit (CD117), M-CSF (macrophage colony-stimulating factor) receptor (CD115), and IL-7 (interleukin-7) receptor-α (CD127). The CDPs comprise of two progenitors, CD115(+) CDPs and CD115(-) CDPs, and give rise to only DC subsets in both in vitro and in vivo. The former CDPs are the main source of cDC, while the later CDPs are the main source of pDC in vivo. Here, we provide a protocol for the isolation of dendritic cell progenitor and bone marrow progenitor cells from mouse.

摘要

在稳态淋巴器官中，树突状细胞（DC）包括两个主要亚群，即传统DC（cDC）和浆细胞样DC（pDC）。这些细胞半衰期短，因此需要从造血干细胞和祖细胞持续生成。最近，我们在小鼠骨髓中鉴定出了称为普通DC祖细胞（CDP）的DC限制性祖细胞。CDP可基于造血细胞因子受体从小鼠骨髓中分离出来，这些受体如Flt3（Fms相关酪氨酸激酶3）（CD135）、c-kit（CD117）、M-CSF（巨噬细胞集落刺激因子）受体（CD115）和IL-7（白细胞介素-7）受体-α（CD127）。CDP由两个祖细胞组成，即CD115(+) CDP和CD115(-) CDP，在体外和体内都仅产生DC亚群。前者CDP是cDC的主要来源，而后者CDP是体内pDC的主要来源。在这里，我们提供了从小鼠中分离树突状细胞祖细胞和骨髓祖细胞的方案。