卵形鲳鲹c型溶菌酶基因的克隆与表达分析
Cloning and expression analysis of c-type lysozyme gene in golden pompano, Trachinotus ovatus.
作者信息
Zhou Chuanpeng, Lin Heizhao, Huang Zhong, Wang Jun, Wang Yun, Yu Wei
机构信息
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, PR China; Guangdong Provincial Key Laboratory of Applied Marine Biology, PR China.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, PR China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen, 518121, PR China.
出版信息
Fish Shellfish Immunol. 2016 Jul;54:580-5. doi: 10.1016/j.fsi.2016.04.137. Epub 2016 May 2.
It is well known that lysozymes are key proteins to teleosts in the innate immune system and possess high bactericidal properties. In the present study, a c-type lysozyme gene (To-lysC) was cloned from golden pompano, Trachinotus ovatus. The To-lysC cDNA is composed of 743 bp with a 36 bp of 5'-UTR, 432 bp open reading frame (ORF) and 275 bp 3'-UTR, encoding a polypeptide of 144 amino acids (GenBank accession no: KT935522). Phylogenetic analysis revealed that To-lysC showed highest similarity to Perca flavescens lysC. Quantitative real-time PCR (qRT-PCR) analysis showed that To-lysC had relatively high expression level in the head kidney, gill and brain. After Vibrio harveyi infection, transcripts of To-lysC increased and reached its peak at 12 h p.i. These results indicated that To-lysC may play an important role in innate immune response to bacteria.
众所周知，溶菌酶是硬骨鱼先天免疫系统中的关键蛋白质，具有很高的杀菌特性。在本研究中，从卵形鲳鲹（Trachinotus ovatus）中克隆了一个c型溶菌酶基因（To-lysC）。To-lysC cDNA由743 bp组成，其中5'-UTR为36 bp，开放阅读框（ORF）为432 bp，3'-UTR为275 bp，编码一个144个氨基酸的多肽（GenBank登录号：KT935522）。系统发育分析表明，To-lysC与黄鲈（Perca flavescens）lysC的相似性最高。实时定量PCR（qRT-PCR）分析表明，To-lysC在头肾、鳃和脑中具有相对较高的表达水平。哈维氏弧菌感染后，To-lysC的转录本增加，并在感染后12小时达到峰值。这些结果表明，To-lysC可能在对细菌的先天免疫反应中发挥重要作用。
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