三种检测丙型肝炎病毒患者IL28 rs12979860多态性作为治疗结果预测指标的不同方法的比较
Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus.
作者信息
Fateh Abolfazl, Aghasadeghi Mohammadreza, Siadat Seyed D, Vaziri Farzam, Sadeghi Farzin, Fateh Roohollah, Keyvani Hossein, Tasbiti Alireza H, Yari Shamsi, Ataei-Pirkooh Angila, Monavari Seyed H
机构信息
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
出版信息
Osong Public Health Res Perspect. 2016 Apr;7(2):83-9. doi: 10.1016/j.phrp.2015.11.004. Epub 2015 Nov 30.
OBJECTIVES
This study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C.
METHODS
A total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR.
RESULTS
The different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6%), 35(68.6%), and six (11.8%), respectively, and in HCV genotype 31, it was 13(26.5%), 31(63.3%), and five (10.2%), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710).
CONCLUSION
Screening by ARMS - PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice.
目的
本研究旨在评估三种基因多态性分析方法的特异性、敏感性、成本和周转时间，并研究慢性丙型肝炎患者中白细胞介素28B（IL28B）rs12979860与聚乙二醇干扰素-α/利巴韦林（pegIFN-α/RVB）治疗的持续病毒学应答率（SVR）之间的关系。
方法
使用直接测序、实时聚合酶链反应（PCR）、扩增阻滞突变系统（ARMS）-PCR这三种方法对100例慢性丙型肝炎患者的样本进行平行分析。
结果
通过PCR-限制性片段长度多态性分析（RFLP）、ARMS-PCR和直接测序获得的IL28B rs12979860等位基因（CC、CT和TT）的不同图谱在这三种方法中是一致的。在丙型肝炎病毒（HCV）1a基因型中，rs12979860基因型CC、CT和TT的患病率分别为10例（19.6%）、35例（68.6%）和6例（11.8%），在HCV 3型中，分别为13例（26.5%）、31例（63.3%）和5例（10.2%）。rs12979860基因型与HCV基因型之间未见显著差异（p = 0.710）。
结论
在常规临床实践中，通过ARMS-PCR SNOP检测进行筛查是确定HCV多态性最有效、最可靠的方法。
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