Nakao Shintaro, Hafezi-Moghadam Ali
Center for Excellence in Functional and Molecular Imaging, Brigham and Women's Hospital, 75 Francis Street, Boston, MA, 02115, USA.
Department of Radiology, Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
Methods Mol Biol. 2016;1430:311-6. doi: 10.1007/978-1-4939-3628-1_21.
The cornea is a transparent tissue that lacks blood and lymphatic vessels. In addition, the cornea is readily accessible, which makes it convenient for direct visualization of angiogenesis and lymphangiogenesis. The corneal micropocket assay is a commonly used quantitative technique, in which a growth factor containing pellet is micro-surgically implanted into the cornea of a rodent. Subsequently, the growth of the preexisting limbal vessels toward the growth factor is visualized by live microscopy or immunohistochemistry. Recently, there has been significant interest in the process of lymphangiogenesis and the factors that regulate it. To facilitate these studies, we introduce a novel technique for visualization of the immune response during growth factor induced angiogenesis and lymphangiogenesis in the cornea.
角膜是一种缺乏血管和淋巴管的透明组织。此外，角膜易于触及，这使得直接观察血管生成和淋巴管生成变得方便。角膜微袋试验是一种常用的定量技术，其中将含有生长因子的小球通过显微手术植入啮齿动物的角膜。随后，通过活体显微镜或免疫组织化学观察先前存在的角膜缘血管向生长因子的生长情况。最近，人们对淋巴管生成过程及其调控因素产生了浓厚兴趣。为了促进这些研究，我们引入了一种新技术，用于观察生长因子诱导的角膜血管生成和淋巴管生成过程中的免疫反应。
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