Carpagnano Giovanna E, Malerba Mario, Lacedonia Donato, Susca Antonia, Logrieco Antonio, Carone Mauro, Cotugno Grazia, Palmiotti Giuseppe A, Foschino-Barbaro Maria P
Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Italy.
Allergy Asthma Proc. 2016 May;37(3):41-6. doi: 10.2500/aap.2016.37.3943.
The presence of virus and bacteria in the airways of subjects with asthma is common and seems to be associated with a deterioration due to the disease. The microbiologic study of airways in asthma is foreseen by guidelines with induced sputum that is often ineffective and contraindicated in severe asthma.
To analyze the fungal microbiome in the exhaled breath condensate (EBC) of subjects with asthma by evaluating a possible correlation with anthropometric and asthma severity data.
We enrolled 47 consecutive subjects with asthma (28 with atopic asthma and 19 with nonatopic asthma) and 20 controls. Enrolled subjects underwent EBC and sputum collection. Fungal microbiome was assessed by culture on EBC and sputum samples by using Czapek yeast extract agar.
A fungal colonization in the EBC of 70% of enrolled subjects with asthma was detected (none detected in the controls). An overlap of fungal microbiome in EBC and sputum was observed (100% of overlap). Fungal colonization was higher in subjects without atopic, obesity, and severe and uncontrolled asthma.
When considering the high morbidity and mortality of patients with severe asthma in whom we found an important fungal airways colonization, we support the use of the analysis of exhaled fungal microbiome in these subjects.
哮喘患者气道中病毒和细菌的存在很常见，似乎与疾病导致的病情恶化有关。哮喘气道的微生物学研究可通过诱导痰进行，这在指南中有规定，但诱导痰在严重哮喘中往往无效且禁忌。
通过评估与人体测量学和哮喘严重程度数据的可能相关性，分析哮喘患者呼出气冷凝液（EBC）中的真菌微生物群。
我们连续招募了47名哮喘患者（28名特应性哮喘患者和19名非特应性哮喘患者）和20名对照者。入选的受试者接受了EBC和痰液采集。通过使用察氏酵母提取物琼脂对EBC和痰液样本进行培养来评估真菌微生物群。
在70%的入选哮喘患者的EBC中检测到真菌定植（对照组未检测到）。观察到EBC和痰液中的真菌微生物群存在重叠（重叠率为100%）。在无特应性、肥胖以及严重和未控制哮喘的患者中，真菌定植率更高。
鉴于我们发现严重哮喘患者存在重要的气道真菌定植，其发病率和死亡率较高，我们支持对这些患者进行呼出气真菌微生物群分析。
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