乌干达拉凯地区的生殖器厌氧细菌过度生长与PrePex男性包皮环切器械
Genital Anaerobic Bacterial Overgrowth and the PrePex Male Circumcision Device, Rakai, Uganda.
作者信息
Liu Cindy M, Prodger Jessica L, Tobian Aaron A R, Serwadda David, Galiwango Ronald M, Nalugoda Fred, Kighoma Nehemiah, Mwinike Joshua, Anyokorit Margaret, Price Lance B, Wawer Maria J, Kigozi Godfrey, Gray Ronald H
机构信息
Department of Pathology, School of Medicine Center for Microbial Genetics and Genomics, Northern Arizona University Division of Pathogen Genomics, Translational Genomics Research Institute, Flagstaff, Arizona.
Department of Epidemiology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland.
出版信息
J Infect Dis. 2016 Aug 15;214(4):595-8. doi: 10.1093/infdis/jiw182. Epub 2016 May 13.
The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene-based quantitative polymerase chain reaction analysis and sequencing. PrePex users had higher absolute abundance of all bacteria than uncircumcised men (P = .001), largely due to increased numbers of the following anaerobes: Porphyromonas (5.2 × 10(7) 16S rRNA gene copies/swab in the PrePex group and 1.1 × 10(6) 16S rRNA gene copies/swab in uncircumcised men; P = .002), Peptoniphilus (1.0 × 10(7) and 1.8 × 10(6) 16S rRNA gene copies/swab, respectively; P < .05), Anaerococcus (1.0 × 10(7) and 1.1 × 10(6) 16S rRNA gene copies/swab, respectively; P < .001), and Campylobacter ureolyticus (1.7 × 10(5) and 1.6 × 10(7)16S rRNA gene copies/swab, respectively; P < .001). The PrePex-associated increase in anaerobes may account for unpleasant odor and a possible heightened risk of tetanus.
PrePex包皮环切器械会导致包皮缺血性坏死，引发了对厌氧菌过度生长的担忧。我们使用基于16S核糖体（rRNA）基因的定量聚合酶链反应分析和测序，比较了在乌干达拉凯地区，2名使用PrePex器械7天后的男性与145名未行包皮环切术男性的包皮下微生物群。使用PrePex器械的男性所有细菌的绝对丰度均高于未行包皮环切术的男性（P = 0.001），这主要是由于以下厌氧菌数量增加：卟啉单胞菌（PrePex组每拭子16S rRNA基因拷贝数为5.2×10⁷，未行包皮环切术男性每拭子为1.1×10⁶；P = 0.002）、嗜胨菌（分别为每拭子1.0×10⁷和1.8×10⁶个16S rRNA基因拷贝；P <0.05）、厌氧球菌（分别为每拭子1.0×10⁷和1.1×10⁶个16S rRNA基因拷贝；P <0.001）和解脲弯曲菌（分别为每拭子1.7×10⁵和1.6×10⁷个16S rRNA基因拷贝；P <0.001）。PrePex相关的厌氧菌增加可能导致异味，并可能增加破伤风风险。
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