在非流行地区，与溶组织内阿米巴的传统检测和核酸检测相比，抗原检测的诊断性能。

Diagnostic performances of antigen detection compared to conventional and nucleic acid detection of Entamoeba histolytica in a non-endemic setting.

作者信息

Calderaro Adriana, Piergianni Maddalena, Piccolo Giovanna, Rossi Sabina, Montecchini Sara, Buttrini Mirko, Arcangeletti Maria Cristina, Medici Maria Cristina, Chezzi Carlo, De Conto Flora

机构信息

Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.

出版信息

New Microbiol. 2016 Apr;39(2):153-5.

PMID:27196557

Abstract

This study evaluated the immunochromatographic (IC) assay "TECHLAB(®) E. HISTOLYTICA QUIK CHEK™" analysing 36 faecal samples and 7 cultured strains. This assay was compared to the methods performed in our laboratory for the diagnosis of amoebiasis. The IC assay revealed a detection limit of 103 trophozoites/g faeces and no cross-reactivity with other parasites and failed to detect E. histolytica antigen in frozen faeces. In our laboratory located in a non-endemic setting this assay could not replace the methods currently used for the diagnosis of amoebiasis.

摘要

本研究评估了免疫层析（IC）检测法“TECHLAB(®) 溶组织内阿米巴快速检测™”，分析了36份粪便样本和7株培养菌株。该检测法与我们实验室用于诊断阿米巴病的方法进行了比较。IC检测法显示检测限为每克粪便103个滋养体，与其他寄生虫无交叉反应，且未能在冷冻粪便中检测到溶组织内阿米巴抗原。在我们位于非流行地区的实验室，该检测法无法替代目前用于诊断阿米巴病的方法。