Bircan Burak, Çakır Murat, Kırbağ Sevda, Gül Hüseyin Fatih
a Biology Department, Faculty of Science , Fırat Universitesi , Elazig , Turkey ;
b Physiology Department, Faculty of Medicine , Inonu University , Malatya , Turkey ;
Ren Fail. 2016 Aug;38(7):1122-8. doi: 10.1080/0886022X.2016.1184957. Epub 2016 May 19.
Apelin is a peptide hormone defined as a ligand for G-protein clamped receptor (APJ) receptor. It is indicated in the literature both apelin and APJ are synthesized on the peripheral tissues including the renal tissues. Which roles does the apelin play on the renal tissue has not been completely illuminated yet. This study is designed to determine the possible protective effect of apelin-13 on the kidney I/R injury. Adult male Sprague-Dawley rats were used in this study. In the sham group, right kidneys of the animals were dissected. In the I/R group, right kidney was dissected and ischemia of 45 min was performed, and then reperfusion was applied for 3 h. In the treatment groups, three different doses of apelin were injected at the beginning of the ischemia unlike the I/R group. BUN, Cre, Na, K, Cl, total protein and albumin from serum samples were determined and TNF-α, IL-1β, IL-6, TAS and TOS parameters were read with ELISA reader. MDA, SOD, CAT and GSH-Px enzyme activations from renal tissues were measured. In comparison with the sham and I/R groups, while the serum BUN, CRE, CI and TNF-α levels showed an increase in the groups on which the apelin-13 was applied, Na, total protein, albumin, TAS levels decreased. Serum TOS level of other groups showed an increase by comparison with the sham group. Our results showed that apelin-13 applied after I/R increased the antioxidant enzyme activity in a dose dependent manner, prevented the lipid oxidation and improved the renal functions.
阿片肽是一种肽类激素，被定义为G蛋白偶联受体（APJ）的配体。文献表明，阿片肽和APJ均在外周组织（包括肾组织）中合成。阿片肽在肾组织中发挥何种作用尚未完全阐明。本研究旨在确定阿片肽-13对肾脏缺血/再灌注损伤可能的保护作用。本研究使用成年雄性Sprague-Dawley大鼠。假手术组中，解剖动物的右肾。缺血/再灌注组中，解剖右肾并进行45分钟的缺血处理，然后进行3小时的再灌注。与缺血/再灌注组不同，治疗组在缺血开始时注射三种不同剂量的阿片肽。测定血清样本中的尿素氮、肌酐、钠、钾、氯、总蛋白和白蛋白，并使用酶标仪读取肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6、总抗氧化能力和总氧化应激参数。测量肾组织中的丙二醛、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。与假手术组和缺血/再灌注组相比，应用阿片肽-13的组中血清尿素氮、肌酐、氯和肿瘤坏死因子-α水平升高，而钠、总蛋白、白蛋白、总抗氧化能力水平降低。与假手术组相比，其他组的血清总氧化应激水平升高。我们的结果表明，缺血/再灌注后应用阿片肽-13以剂量依赖的方式增加抗氧化酶活性，防止脂质氧化并改善肾功能。
