Sun Tongjun, Zhang Yaxi, Li Yan, Zhang Qian, Ding Yuli, Zhang Yuelin
Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
National Institute of Biological Sciences, Beijing, 102206, China.
Nat Commun. 2015 Dec 18;6:10159. doi: 10.1038/ncomms10159.
Recognition of pathogens by host plants leads to rapid transcriptional reprogramming and activation of defence responses. The expression of many defence regulators is induced in this process, but the mechanisms of how they are controlled transcriptionally are largely unknown. Here we use chromatin immunoprecipitation sequencing to show that the transcription factors SARD1 and CBP60g bind to the promoter regions of a large number of genes encoding key regulators of plant immunity. Among them are positive regulators of systemic immunity and signalling components for effector-triggered immunity and PAMP-triggered immunity, which is consistent with the critical roles of SARD1 and CBP60g in these processes. In addition, SARD1 and CBP60g target a number of genes encoding negative regulators of plant immunity, suggesting that they are also involved in negative feedback regulation of defence responses. Based on these findings we propose that SARD1 and CBP60g function as master regulators of plant immune responses.
宿主植物对病原体的识别会导致快速的转录重编程和防御反应的激活。在此过程中，许多防御调节因子的表达被诱导，但它们在转录水平上是如何被调控的机制在很大程度上仍未知。在这里，我们使用染色质免疫沉淀测序来表明，转录因子SARD1和CBP60g与大量编码植物免疫关键调节因子的基因的启动子区域结合。其中包括系统性免疫的正调控因子以及效应子触发免疫和模式触发免疫的信号成分，这与SARD1和CBP60g在这些过程中的关键作用是一致的。此外，SARD1和CBP60g还靶向许多编码植物免疫负调控因子的基因，这表明它们也参与防御反应的负反馈调节。基于这些发现，我们提出SARD1和CBP60g作为植物免疫反应的主要调节因子发挥作用。
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