• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

急性和慢性电惊厥发作（ECS）对成年大鼠海马体中表观遗传机制的表达有不同调节作用。

Acute and Chronic Electroconvulsive Seizures (ECS) Differentially Regulate the Expression of Epigenetic Machinery in the Adult Rat Hippocampus.

作者信息

Pusalkar Madhavi, Ghosh Shreya, Jaggar Minal, Husain Basma Fatima Anwar, Galande Sanjeev, Vaidya Vidita A

机构信息

Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India (Dr Pusalkar, Ms Ghosh, Ms Jaggar, Ms Husain, and Dr Vaidya); Centre of Excellence in Epigenetics, Indian Institute of Science Education and Research, Pune, Maharashtra, India (Dr Galande).

出版信息

Int J Neuropsychopharmacol. 2016 Sep 21;19(9). doi: 10.1093/ijnp/pyw040. Print 2016 Sep.

DOI:10.1093/ijnp/pyw040
PMID:27207907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5043647/
Abstract

BACKGROUND

Electroconvulsive seizure treatment is a fast-acting antidepressant therapy that evokes rapid transcriptional, neurogenic, and behavioral changes. Epigenetic mechanisms contribute to altered gene regulation, which underlies the neurogenic and behavioral effects of electroconvulsive seizure. We hypothesized that electroconvulsive seizure may modulate the expression of epigenetic machinery, thus establishing potential alterations in the epigenetic landscape.

METHODS

We examined the influence of acute and chronic electroconvulsive seizure on the gene expression of histone modifiers, namely histone acetyltransferases, histone deacetylases, histone methyltransferases, and histone (lysine) demethylases as well as DNA modifying enzymes, including DNA methyltransferases, DNA demethylases, and methyl-CpG-binding proteins in the hippocampi of adult male Wistar rats using quantitative real time-PCR analysis. Further, we examined the influence of acute and chronic electroconvulsive seizure on global and residue-specific histone acetylation and methylation levels within the hippocampus, a brain region implicated in the cellular and behavioral effects of electroconvulsive seizure.

RESULTS

Acute and chronic electroconvulsive seizure induced a primarily unique, and in certain cases bidirectional, regulation of histone and DNA modifiers, and methyl-CpG-binding proteins, with an overlapping pattern of gene regulation restricted to Sirt4, Mll3, Jmjd3, Gadd45b, Tet2, and Tet3. Global histone acetylation and methylation levels were predominantly unchanged, with the exception of a significant decline in H3K9 acetylation in the hippocampus following chronic electroconvulsive seizure.

CONCLUSIONS

Electroconvulsive seizure treatment evokes the transcriptional regulation of several histone and DNA modifiers, and methyl-CpG-binding proteins within the hippocampus, with a predominantly distinct pattern of regulation induced by acute and chronic electroconvulsive seizure.

摘要

背景

电惊厥治疗是一种起效迅速的抗抑郁疗法，可引发快速的转录、神经发生及行为变化。表观遗传机制参与基因调控的改变，这是电惊厥治疗的神经发生和行为效应的基础。我们推测电惊厥可能调节表观遗传机制的表达，从而在表观遗传格局中产生潜在改变。

方法

我们使用定量实时聚合酶链反应分析，检测急性和慢性电惊厥对成年雄性Wistar大鼠海马体中组蛋白修饰因子（即组蛋白乙酰转移酶、组蛋白去乙酰化酶、组蛋白甲基转移酶和组蛋白（赖氨酸）去甲基化酶）以及DNA修饰酶（包括DNA甲基转移酶、DNA去甲基化酶和甲基-CpG结合蛋白）基因表达的影响。此外，我们还检测了急性和慢性电惊厥对海马体中整体及残基特异性组蛋白乙酰化和甲基化水平的影响，海马体是一个与电惊厥治疗的细胞和行为效应相关的脑区。

结果

急性和慢性电惊厥主要诱导了独特的，在某些情况下是双向的组蛋白和DNA修饰因子以及甲基-CpG结合蛋白的调控，基因调控的重叠模式仅限于沉默调节蛋白4、混合谱系白血病蛋白3、含JmjC结构域的组蛋白去甲基化酶3、生长停滞和DNA损伤诱导蛋白45β、DNA去甲基化酶2和DNA去甲基化酶3。除了慢性电惊厥后海马体中H3K9乙酰化显著下降外，整体组蛋白乙酰化和甲基化水平基本未变。

结论

电惊厥治疗可引发海马体内多种组蛋白和DNA修饰因子以及甲基-CpG结合蛋白的转录调控，急性和慢性电惊厥诱导的调控模式主要不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/62b85f683976/ijnppy_pyw040_f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/08c8f36ab800/ijnppy_pyw040_f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/52e0b6a7cc30/ijnppy_pyw040_f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/4cf0ab28b8c8/ijnppy_pyw040_f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/20a71cf20820/ijnppy_pyw040_f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/62b85f683976/ijnppy_pyw040_f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/08c8f36ab800/ijnppy_pyw040_f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/52e0b6a7cc30/ijnppy_pyw040_f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/4cf0ab28b8c8/ijnppy_pyw040_f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/20a71cf20820/ijnppy_pyw040_f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f81e/5043647/62b85f683976/ijnppy_pyw040_f0005.jpg

相似文献

1
Acute and Chronic Electroconvulsive Seizures (ECS) Differentially Regulate the Expression of Epigenetic Machinery in the Adult Rat Hippocampus.急性和慢性电惊厥发作（ECS）对成年大鼠海马体中表观遗传机制的表达有不同调节作用。
Int J Neuropsychopharmacol. 2016 Sep 21;19(9). doi: 10.1093/ijnp/pyw040. Print 2016 Sep.
2
Early stress evokes dysregulation of histone modifiers in the medial prefrontal cortex across the life span.早期应激会在整个生命周期中引发内侧前额叶皮质中组蛋白修饰因子的失调。
Dev Psychobiol. 2016 Mar;58(2):198-210. doi: 10.1002/dev.21365. Epub 2015 Sep 23.
3
Epigenetic landscape of amphetamine and methamphetamine addiction in rodents.啮齿动物中苯丙胺和甲基苯丙胺成瘾的表观遗传格局
Epigenetics. 2015;10(7):574-80. doi: 10.1080/15592294.2015.1055441.
4
Developmental exposure to 50 parts-per-billion arsenic influences histone modifications and associated epigenetic machinery in a region- and sex-specific manner in the adult mouse brain.成年小鼠大脑中，发育阶段暴露于五十亿分之一的砷会以区域和性别特异性的方式影响组蛋白修饰及相关表观遗传机制。
Toxicol Appl Pharmacol. 2015 Oct 1;288(1):40-51. doi: 10.1016/j.taap.2015.07.013. Epub 2015 Jul 17.
5
Uteroplacental insufficiency affects epigenetic determinants of chromatin structure in brains of neonatal and juvenile IUGR rats.子宫胎盘功能不全影响新生和幼年宫内生长受限大鼠大脑中染色质结构的表观遗传决定因素。
Physiol Genomics. 2006 Mar 13;25(1):16-28. doi: 10.1152/physiolgenomics.00093.2005. Epub 2005 Dec 27.
6
Repeated treatment with electroconvulsive seizures induces HDAC2 expression and down-regulation of NMDA receptor-related genes through histone deacetylation in the rat frontal cortex.反复进行电惊厥治疗可通过大鼠额叶皮质中的组蛋白去乙酰化诱导HDAC2表达并下调NMDA受体相关基因。
Int J Neuropsychopharmacol. 2014 Sep;17(9):1487-500. doi: 10.1017/S1461145714000248. Epub 2014 Mar 10.
7
Influence of Chronic Electroconvulsive Seizures on Plasticity-Associated Gene Expression and Perineuronal Nets Within the Hippocampi of Young Adult and Middle-Aged Sprague-Dawley Rats.慢性电抽搐对青年和中年 Sprague-Dawley 大鼠海马内可塑性相关基因表达和神经周细胞网络的影响。
Int J Neuropsychopharmacol. 2023 Apr 17;26(4):294-306. doi: 10.1093/ijnp/pyad008.
8
Social isolation-induced epigenetic changes in midbrain of adult mice.社会隔离诱导成年小鼠中脑的表观遗传变化。
J Physiol Pharmacol. 2014 Apr;65(2):247-55.
9
Epigenetic targeting of histone deacetylase: therapeutic potential in Parkinson's disease?组蛋白去乙酰化酶的表观遗传学靶向：帕金森病的治疗潜力？
Pharmacol Ther. 2013 Oct;140(1):34-52. doi: 10.1016/j.pharmthera.2013.05.010. Epub 2013 May 24.
10
Histone methylation patterns in astrocytes are influenced by age following ischemia.缺血后星形胶质细胞中的组蛋白甲基化模式受年龄影响。
Epigenetics. 2015;10(2):142-52. doi: 10.1080/15592294.2014.1001219. Epub 2015 Feb 3.

引用本文的文献

1
Exome sequencing of 20,979 individuals with epilepsy reveals shared and distinct ultra-rare genetic risk across disorder subtypes.对 20979 名癫痫患者进行外显子组测序，揭示了不同疾病亚型之间共享和独特的超罕见遗传风险。
Nat Neurosci. 2024 Oct;27(10):1864-1879. doi: 10.1038/s41593-024-01747-8. Epub 2024 Oct 3.
2
A systematic mini-review of epigenetic mechanisms associated with electroconvulsive therapy in humans.关于人类电休克治疗相关表观遗传机制的系统性小型综述。
Front Hum Neurosci. 2023 Mar 9;17:1143332. doi: 10.3389/fnhum.2023.1143332. eCollection 2023.
3
Exome sequencing of 20,979 individuals with epilepsy reveals shared and distinct ultra-rare genetic risk across disorder subtypes.

本文引用的文献

1
BDNF - a key transducer of antidepressant effects.脑源性神经营养因子——抗抑郁作用的关键转导因子。
Neuropharmacology. 2016 Mar;102:72-9. doi: 10.1016/j.neuropharm.2015.10.034. Epub 2015 Nov 11.
2
A Novel Function of TET2 in CNS: Sustaining Neuronal Survival.TET2在中枢神经系统中的新功能：维持神经元存活。
Int J Mol Sci. 2015 Sep 10;16(9):21846-57. doi: 10.3390/ijms160921846.
3
Gadd45b Mediates Electroconvulsive Shock Induced Proliferation of Hippocampal Neural Stem Cells.Gadd45b介导电休克诱导的海马神经干细胞增殖。
对20979名癫痫患者进行外显子组测序，揭示了不同癫痫亚型中共同存在和独特的超罕见遗传风险。
medRxiv. 2024 Sep 20:2023.02.22.23286310. doi: 10.1101/2023.02.22.23286310.
4
Prelimbic Cortical Stimulation with L-methionine Enhances Cognition through Hippocampal DNA Methylation and Neuroplasticity Mechanisms.用L-甲硫氨酸进行边缘前皮质刺激通过海马体DNA甲基化和神经可塑性机制增强认知。
Aging Dis. 2023 Feb 1;14(1):112-135. doi: 10.14336/AD.2022.0706.
5
Decoding the Mechanism of Action of Rapid-Acting Antidepressant Treatment Strategies: Does Gender Matter?解码快速抗抑郁治疗策略的作用机制：性别因素重要吗？
Int J Mol Sci. 2019 Feb 22;20(4):949. doi: 10.3390/ijms20040949.
6
Volume of the Human Hippocampus and Clinical Response Following Electroconvulsive Therapy.人类海马体体积与电抽搐治疗后的临床反应。
Biol Psychiatry. 2018 Oct 15;84(8):574-581. doi: 10.1016/j.biopsych.2018.05.017. Epub 2018 May 29.
7
Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins.大鼠的电惊厥发作及其海马体分离以检查发作诱导的突触后密度蛋白变化。
J Vis Exp. 2017 Aug 15(126):56016. doi: 10.3791/56016.
8
How Electroconvulsive Therapy Works?: Understanding the Neurobiological Mechanisms.电休克疗法是如何起作用的？：理解神经生物学机制。
Clin Psychopharmacol Neurosci. 2017 Aug 31;15(3):210-221. doi: 10.9758/cpn.2017.15.3.210.
Brain Stimul. 2015 Nov-Dec;8(6):1021-1024. doi: 10.1016/j.brs.2015.07.036. Epub 2015 Jul 28.
4
Kdm6b and Pmepa1 as Targets of Bioelectrically and Behaviorally Induced Activin A Signaling.Kdm6b 和 Pmepa1 作为生物电和行为诱导的激活素 A 信号的靶标。
Mol Neurobiol. 2016 Aug;53(6):4210-4225. doi: 10.1007/s12035-015-9363-3. Epub 2015 Jul 28.
5
Epigenetic regulation of Dnmt3a and Arc gene expression after electroconvulsive stimulation in the rat.大鼠电惊厥刺激后Dnmt3a和Arc基因表达的表观遗传调控
Mol Cell Neurosci. 2015 Jul;67:137-43. doi: 10.1016/j.mcn.2015.06.011. Epub 2015 Jun 30.
6
Tet3 regulates synaptic transmission and homeostatic plasticity via DNA oxidation and repair.Tet3通过DNA氧化和修复调节突触传递和稳态可塑性。
Nat Neurosci. 2015 Jun;18(6):836-43. doi: 10.1038/nn.4008. Epub 2015 Apr 27.
7
Expression of DNMT1 in neural stem/precursor cells is critical for survival of newly generated neurons in the adult hippocampus.DNMT1在神经干/前体细胞中的表达对于成年海马体中新生成神经元的存活至关重要。
Neurosci Res. 2015 Jun;95:1-11. doi: 10.1016/j.neures.2015.01.014. Epub 2015 Feb 4.
8
Activation of neuronal gene expression by the JMJD3 demethylase is required for postnatal and adult brain neurogenesis.JMJD3去甲基化酶激活神经元基因表达是出生后及成体脑神经元发生所必需的。
Cell Rep. 2014 Sep 11;8(5):1290-9. doi: 10.1016/j.celrep.2014.07.060. Epub 2014 Aug 28.
9
HDAC3 controls gap 2/mitosis progression in adult neural stem/progenitor cells by regulating CDK1 levels.组蛋白去乙酰化酶3通过调节细胞周期蛋白依赖性激酶1的水平来控制成年神经干细胞/祖细胞中G2期/有丝分裂进程。
Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13541-6. doi: 10.1073/pnas.1411939111. Epub 2014 Aug 26.
10
Histone deacetylase 8 suppresses osteogenic differentiation of bone marrow stromal cells by inhibiting histone H3K9 acetylation and RUNX2 activity.组蛋白去乙酰化酶8通过抑制组蛋白H3K9乙酰化和RUNX2活性来抑制骨髓基质细胞的成骨分化。
Int J Biochem Cell Biol. 2014 Sep;54:68-77. doi: 10.1016/j.biocel.2014.07.003. Epub 2014 Jul 11.