Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
J Adv Res. 2016 May;7(3):491-8. doi: 10.1016/j.jare.2016.03.008. Epub 2016 Mar 29.
MicroRNAs (miRNAs) play an important role in regulating gene expression at the post-transcriptional level and are involved in numerous physiological processes. Accumulating evidence suggests that single-nucleotide polymorphisms (SNPs) in human miRNA genes may affect miRNA biogenesis pathway and influence the susceptibility to several diseases such as cancer. The present study aimed to evaluate the impact of miR-499 rs3746444, miR-196a2 rs11614913, miR-149 rs2292832, and miR-146a rs2910164 polymorphisms on prostate cancer (PCa) risk in a sample of Iranian population. This case-control study was done on 169 patients with pathologically confirmed PCa and 182 benign prostatic hyperplasia (BPH). The genotyping assays were done using T-ARMS-PCR or PCR-RFLP methods. The findings indicated that CC genotype of miR-499 rs3746444 polymorphism increased the risk of PCa (OR = 1.76, 95% CI = 1.12-2.79, P = 0.019) compared to TT genotype. No statistically significant association was found between miR-196a2 rs11614913, miR-149 rs2292832, and miR-146a rs2910164 polymorphisms and PCa risk. In summary, the findings indicated that miR-499 rs3746444 polymorphism increased the risk of PCa in an Iranian population. Further studies with larger sample sizes and different ethnicities are necessary to verify the findings of the present study.
微小 RNA（miRNA）在后转录水平上调控基因表达中发挥重要作用，并参与许多生理过程。越来越多的证据表明，人类 miRNA 基因的单核苷酸多态性（SNP）可能影响 miRNA 生物发生途径，并影响多种疾病（如癌症）的易感性。本研究旨在评估 miR-499 rs3746444、miR-196a2 rs11614913、miR-149 rs2292832 和 miR-146a rs2910164 多态性对伊朗人群前列腺癌（PCa）风险的影响。这项病例对照研究在 169 例经病理证实的 PCa 患者和 182 例良性前列腺增生（BPH）患者中进行。基因分型检测采用 T-ARMS-PCR 或 PCR-RFLP 方法。研究结果表明，与 TT 基因型相比，miR-499 rs3746444 多态性的 CC 基因型增加了 PCa 的风险（OR=1.76，95%CI=1.12-2.79，P=0.019）。miR-196a2 rs11614913、miR-149 rs2292832 和 miR-146a rs2910164 多态性与 PCa 风险之间无统计学显著相关性。综上所述，miR-499 rs3746444 多态性增加了伊朗人群 PCa 的风险。需要进一步进行更大样本量和不同种族的研究来验证本研究的结果。
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