采用标准化接种技术将内皮细胞接种到聚四氟乙烯血管移植物上。
Endothelial cell seeding on PTFE vascular prostheses using a standardized seeding technique.
作者信息
Gerlach J, Kreusel K M, Schauwecker H H, Bücherl E S
机构信息
Chirurgische Klinik, Universitätsklinikum RV/Charlottenburg, Freie Universität Berlin, West Germany.
出版信息
Int J Artif Organs. 1989 Apr;12(4):270-5.
A standardized method was developed for seeding endothelial cells (EC) in tubular vascular grafts. A rotational cell seeding device for tubular prostheses is presented and parameters influencing the kinetics of cell adhesion (rotation speed, graft diameter, cell suspension level, inoculated cell number) are reported. Seeding EC in 14 mm ID PTFE vascular grafts with rotation rate of 10 rph gave an adhesion rate of 80% in a homogeneous monolayer.
开发了一种在管状血管移植物中接种内皮细胞（EC）的标准化方法。介绍了一种用于管状假体的旋转细胞接种装置，并报告了影响细胞黏附动力学的参数（转速、移植物直径、细胞悬液水平、接种细胞数量）。在内径为14mm的聚四氟乙烯血管移植物中以10转/小时的转速接种内皮细胞，可在均匀单层中实现80%的黏附率。
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