对WT1编码的信使核糖核酸进行工程改造以提高树突状细胞中的翻译效率。

Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cells.

作者信息

Benteyn Daphné, Heirman Carlo, Thielemans Kris, Bonehill Aude

机构信息

Laboratory of Molecular and Cellular Therapy, Department of Immunology-Physiology and the Dendritic Cell Bank, Vrije Universiteit Brussel, Laarbeeklaan 103/E235, 1090, Brussels, Belgium.

出版信息

Methods Mol Biol. 2016;1428:115-23. doi: 10.1007/978-1-4939-3625-0_7.

DOI:10.1007/978-1-4939-3625-0_7

PMID:27236795

Abstract

Dendritic cells (DCs) are the orchestrators of the immune system and are frequently used in clinical trials in order to boost the immune system in cancer patients. Among several available techniques for DC modification, mRNA electroporation is an interesting technique due to the favorable characteristics of mRNA. Antigen expression level and duration can be increased by multiple optimizations of an antigen-encoding mRNA template. Here, we describe different molecular modifications to a WT1-encoding mRNA construct in order to increase antigen expression and the subsequent introduction of mRNA into DCs.

摘要

树突状细胞（DCs）是免疫系统的协调者，常用于临床试验以增强癌症患者的免疫系统。在几种可用的DC修饰技术中，mRNA电穿孔是一种有趣的技术，因为mRNA具有良好的特性。通过对抗原编码mRNA模板进行多次优化，可以提高抗原表达水平和持续时间。在这里，我们描述了对WT1编码mRNA构建体的不同分子修饰，以增加抗原表达并随后将mRNA导入DCs。