小龙虾视叶中的乙酰胆碱：浓度分布及细胞定位。
Acetylcholine in the crayfish optic lobe: concentration profile and cellular localization.
作者信息
Wang-Bennett L T, Pfeiffer C, Arnold J, Glantz R M
机构信息
Department of Biology, Rice University, Houston, Texas 77251.
出版信息
J Neurosci. 1989 Jun;9(6):1864-71. doi: 10.1523/JNEUROSCI.09-06-01864.1989.
The crayfish optic lobe contains high levels of acetylcholine (ACh) and choline as measured with a chemiluminescent assay in small fragments of optic lobe tissue. The highest concentrations were found in the medulla externa and medulla interna (second and third optic neuromeres), which have ACh concentrations of 270 pmol/mg tissue. This concentration is about 16 times that measured in the photoreceptors and lamina ganglionaris (the first optic neuromere). Immunocytochemistry (based upon antisera to choline-glutaryl-BSA) revealed low levels of ACh-like reactivity in the lamina ganglionaris associated with the terminal arbors of centrifugal and/or tangential neurons. The most intense ACh-like reactivity was observed in monopolar neurons of the medulla externa and medulla interna. One monopolar neuron/medullary column (or about 2500 neurons/medullary neuropile) exhibited reactivity and an estimated cytoplasmic concentration of 8.1 mM.
用化学发光分析法对小龙虾视叶组织小片段进行测量，结果显示其中乙酰胆碱（ACh）和胆碱含量很高。在外髓和内髓（第二和第三视神经元节）中发现了最高浓度，其ACh浓度为270 pmol/mg组织。该浓度约为光感受器和神经节层（第一视神经元节）中测得浓度的16倍。免疫细胞化学（基于抗胆碱 - 戊二酰 - BSA血清）显示，与离心和/或切向神经元的终末分支相关的神经节层中，ACh样反应性水平较低。在外髓和内髓的单极神经元中观察到最强的ACh样反应性。一个单极神经元/髓质柱（或约2500个神经元/髓质神经纤维网）表现出反应性，估计细胞质浓度为8.1 mM。
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