胃癌和结直肠癌中17p13杂合性缺失的初步研究。
Preliminary study on the loss of heterozygosity at 17p13 in gastric and colorectal cancers.
作者信息
Wu G J, Shan X N, Li M F, Shi S L, Zheng Q P, Yu L, Zhao S Y
机构信息
Guo-Jun Wu, Shao-Lin Shi, Qi-Ping Zheng, Long Yu, Shou-Yuan Zhao, Institute of Genetics, Fudan University, Shanghai 200433, China.
出版信息
World J Gastroenterol. 1997 Sep 15;3(3):160-2. doi: 10.3748/wjg.v3.i3.160.
AIM
To evaluate the role of p53 in the development and progression of colorectal cancer and gastric carcinoma by analyzing the loss of heterozygosity (LOH) at 17p13.1 and 17p13.3.
METHODS
LOH at the p53 gene locus and 17p13.3 were examined in 22 cases of gastric carcinoma and 14 cases of colorectal cancer by Southern blot analysis.
RESULTS
Of the 22 gastrocarcinoma cases, 12 (54%) were heterozygous and LOH was detected in 6 (50%) of the 12 informative cases. In the 14 colorectal cancer cases, 10 (71%) were heterozygous, and LOH was detected in 6 (60%) of the 10 informative cases.
CONCLUSION
LOH at the p53 gene locus is a frequent event in multiple step carcinogenesis progression. The high frequency of LOH at 17p13.3 suggests that there may be another tumor suppresser gene in that chromosome region.
目的
通过分析17p13.1和17p13.3处的杂合性缺失（LOH）来评估p53在结直肠癌和胃癌发生发展中的作用。
方法
采用Southern印迹分析法检测22例胃癌和14例结直肠癌中p53基因位点及17p13.3处的LOH。
结果
22例胃癌病例中，12例（54%）为杂合子，在12例信息性病例中的6例（50%）检测到LOH。14例结直肠癌病例中，10例（71%）为杂合子，在10例信息性病例中的6例（60%）检测到LOH。
结论
p53基因位点的LOH是多步骤致癌进展中的常见事件。17p13.3处LOH的高频率表明该染色体区域可能存在另一个肿瘤抑制基因。
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