van der Heiden Marieke, van Zelm Menno C, Bartol Sophinus J W, de Rond Lia G H, Berbers Guy A M, Boots Annemieke M H, Buisman Anne-Marie
Centre for Infectious Disease Control (Cib), National Institute for Public Health and the Environment (RIVM), Bilthoven 3720 BA, The Netherlands.
Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Centre Groningen, Groningen 9700 RB, The Netherlands.
Sci Rep. 2016 May 31;6:26892. doi: 10.1038/srep26892.
The elderly population is more susceptible to infections as a result of an altered immune response, commonly referred to as immunosenescence. Cytomegalovirus (CMV)-infection associated changes in blood lymphocytes are known to impact this process, but the interaction with gender remains unclear. Therefore, we analysed the effects and interaction of gender and CMV on the absolute numbers of a comprehensive set of naive and memory T- and B-cell subsets in people between 50 and 65 years of age. Enumeration and characterisation of lymphocyte subsets by flow cytometry was performed on fresh whole blood samples from 255 middle-aged persons. CMV-IgG serostatus was determined by ELISA. Gender was a major factor affecting immune cell numbers. CMV infection was mainly associated with an expansion of late-differentiated T-cell subsets. CMV+ males carried lower numbers of total CD4+, CD4+ central memory (CM) and follicular helper T-cells than females and CMV- males. Moreover, CMV+ males had significantly lower numbers of regulatory T (Treg)-cells and memory B-cells than CMV+ females. We here demonstrate an interaction between the effects of CMV infection and gender on T- and B-cells in middle-aged individuals. These differential effects on adaptive immunity between males and females may have implications for vaccination strategies at middle-age.
由于免疫反应改变，即通常所说的免疫衰老，老年人群更容易受到感染。已知巨细胞病毒（CMV）感染相关的血液淋巴细胞变化会影响这一过程，但与性别的相互作用仍不清楚。因此，我们分析了性别和CMV对50至65岁人群中一整套幼稚和记忆T细胞及B细胞亚群绝对数量的影响及相互作用。通过流式细胞术对来自255名中年人的新鲜全血样本进行淋巴细胞亚群的计数和表征。通过ELISA测定CMV-IgG血清状态。性别是影响免疫细胞数量的主要因素。CMV感染主要与晚期分化T细胞亚群的扩增有关。与女性和CMV阴性男性相比，CMV阳性男性的总CD4 +、CD4 + 中央记忆（CM）和滤泡辅助性T细胞数量更少。此外，CMV阳性男性的调节性T（Treg）细胞和记忆B细胞数量明显低于CMV阳性女性。我们在此证明了CMV感染和性别对中年个体T细胞和B细胞的影响之间存在相互作用。男性和女性在适应性免疫方面的这些差异效应可能对中年人的疫苗接种策略有影响。
