Battista Natalia, Sergi Manuel
Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via 1, Teramo, 64023, Italy.
Methods Mol Biol. 2016;1412:19-26. doi: 10.1007/978-1-4939-3539-0_3.
LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode.2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid, which plays a major role in a wide variety of physiological and pathological processes. Analysis of 2-AG by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy to perform extraction procedures, with reduced solvent consumption.
液相色谱-串联质谱（LC-MS/MS）是一种强大的分析技术，它使用选择反应监测或多反应监测采集模式，能够明确鉴定和可靠定量分析物。2-花生四烯酸甘油酯（2-AG）是最丰富的内源性大麻素，在多种生理和病理过程中起主要作用。通过LC-MS/MS分析2-AG可以检测生物样品中极低的浓度。在此，我们描述了如何通过LC-MS/MS，使用非常快速且易于操作的提取程序，减少溶剂消耗，来测定组织和血浆微量样品中的2-AG水平。
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