虎奶菇（Lignosus rhinocerus）提取物的植物化学特征及其对卵清蛋白诱导的哮喘啮齿动物模型气道炎症的抑制作用。
Phytochemical profiles and inhibitory effects of Tiger Milk mushroom (Lignosus rhinocerus) extract on ovalbumin-induced airway inflammation in a rodent model of asthma.
作者信息
Johnathan M, Gan S H, Ezumi M F Wan, Faezahtul A H, Nurul A A
机构信息
School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
出版信息
BMC Complement Altern Med. 2016 Jun 3;16:167. doi: 10.1186/s12906-016-1141-x.
BACKGROUND
Lignosus rhinocerus (L. rhinocerus), which is known locally as Tiger Milk mushroom, is traditionally used in the treatment of asthma by indigenous communities in Malaysia. However, to date, its efficacy on asthma has not been confirmed by scientific studies and there is also sparse information available on its active constituents. In this study, the volatile constituent of L. rhinocerus hot water extract was investigated using gas chromatography mass spectrometry (GC-MS). The potential effects of L. rhinocerus extract for anti-asthmatic activity was further investigated on ovalbumin (OVA)-sensitized asthmatic Sprague Dawley rats.
METHODS
Sequential extraction using five solvents (petroleum ether, diethyl ether, hexane, ethyl acetate and methanol) was conducted prior to GC-MS analysis. Male Sprague Dawley rats were divided into the following four groups of five animals each: 1) normal rats, 2) sensitization plus OVA-challenged rats 3) sensitization plus OVA-challenged with L. rhinocerus treatment and 4) sensitization plus OVA-challenged with dexamethasone treatment. The levels of immunoglobulin E (IgE) in the serum and T-helper 2 cytokines, including interleukin (IL)-4, IL-5 and IL-13, in bronchoalveolar lavage fluid (BALF), as well as eosinophil infiltration in the lungs, were investigated.
RESULTS
GC-MS analysis revealed the presence of five main groups (alkane, fatty acids, benzene, phenol and dicarboxylic acid) with a total of 18 constituents. Linoleic acid (21.35 %), octadecane (11.82 %) and 2,3-dihydroxypropyl elaidate (10.47 %) were present in high amounts. The extract significantly ameliorated the increase in total IgE in serum and IL-4, IL-5 and IL-13 levels in BALF and also effectively suppressed eosinophils numbers in BALF while attenuating eosinophil infiltrations in the lungs.
CONCLUSION
L. rhinocerus hot water extract has the potential to be used as an alternative for the treatment of acute asthma.
背景
白木香（Lignosus rhinocerus），当地称为虎奶菇，传统上被马来西亚的原住民社区用于治疗哮喘。然而，迄今为止，其对哮喘的疗效尚未得到科学研究的证实，关于其活性成分的信息也很少。在本研究中，使用气相色谱 - 质谱联用仪（GC-MS）对白木香热水提取物的挥发性成分进行了研究。进一步研究了白木香提取物对卵清蛋白（OVA）致敏的哮喘斯普拉格 - 道利大鼠的抗哮喘活性的潜在影响。
方法
在进行GC-MS分析之前，使用五种溶剂（石油醚、乙醚、己烷、乙酸乙酯和甲醇）进行连续萃取。雄性斯普拉格 - 道利大鼠被分为以下四组，每组五只动物：1）正常大鼠，2）致敏加OVA激发大鼠，3）致敏加OVA激发并接受白木香治疗的大鼠，4）致敏加OVA激发并接受地塞米松治疗的大鼠。研究了血清中免疫球蛋白E（IgE）的水平、支气管肺泡灌洗液（BALF）中辅助性T细胞2细胞因子，包括白细胞介素（IL）-4、IL-5和IL-13的水平，以及肺中的嗜酸性粒细胞浸润情况。
结果
GC-MS分析显示存在五个主要类别（烷烃、脂肪酸、苯、苯酚和二羧酸），共有18种成分。亚油酸（21.35%）、十八烷（11.82%）和2,3 - 二羟基丙基反油酸酯（10.47%）含量较高。该提取物显著改善了血清中总IgE的增加以及BALF中IL-4、IL-5和IL-13水平的升高，还有效抑制了BALF中的嗜酸性粒细胞数量，同时减轻了肺中的嗜酸性粒细胞浸润。
结论
白木香热水提取物有潜力用作治疗急性哮喘的替代药物。
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