用于DNA浓缩和纯化的NucleoSpin(®) XS柱。

NucleoSpin(®) XS Columns for DNA Concentration and Clean-Up.

作者信息

Hudlow William R

机构信息

California Department of Justice, Jan Bashinski DNA Laboratory, Richmond, CA, 94804, USA.

California Criminalistics Institute, 11181 Sun Center Drive, Rancho Cordova, CA, 95670, USA.

出版信息

Methods Mol Biol. 2016;1420:125-9. doi: 10.1007/978-1-4939-3597-0_9.

DOI:10.1007/978-1-4939-3597-0_9

PMID:27259735

Abstract

The phenol-chloroform (organic) extraction method continues to be a preferred method for extraction of DNA from forensic evidence samples that may contain low quantities of DNA and polymerase chain reaction (PCR) inhibitors. The aqueous extracts from the organic extraction of DNA require subsequent concentration and cleanup, which has traditionally been performed with microdialysis filter units, including the Centricon(®) and Microcon(®) centrifugal filter devices. Here, we describe the use of the NucleoSpin(®) XS silica columns as an alternative for the concentration and purification of the aqueous extracts from the organic extraction and for the removal of PCR inhibitors from existing DNA extracts.

摘要

苯酚-氯仿（有机）提取法仍然是从可能含有少量DNA和聚合酶链反应（PCR）抑制剂的法医证据样本中提取DNA的首选方法。DNA有机提取后的水相提取物需要后续浓缩和净化，传统上这是通过微透析过滤装置进行的，包括Centricon®和Microcon®离心过滤装置。在此，我们描述了使用NucleoSpin® XS硅胶柱作为替代方法，用于有机提取后水相提取物的浓缩和纯化，以及从现有DNA提取物中去除PCR抑制剂。