Capua I, Minta Z, Karpinska E, Mawditt Karen, Britton P, Cavanagh D, Gough R E
a Istituto Zooprofilattico delle Venezie , Via Romea 14/A, Legnaro , Padova , 35020 , Italy.
b National Veterinary Research Institute , Pulawy , 24-100 , Poland.
Avian Pathol. 1999 Dec;28(6):587-592. doi: 10.1080/03079459994380.
Eighteen isolates of infectious bronchitis virus (IBV) from Italy and Poland in 1997 to 1998 were comprehensively analysed by serum haemagglutination inhibition and virus neutralization tests, and by type-specific polymerase chain reactions and spike protein S1 gene sequencing. Four types of IBV (793/B, 624/I, B1648 and Massachusetts) were detected in Italy, while the presence of 793/B was confirmed in Poland. This showed that not only were four types of IBV co-existing within a single year, but also that several types of IBV have persisted in Europe for many years (at least 13 to 14 years for types B1648 and 793/B). Sequencing of the S1 gene of the 624/I isolate confirmed this as a unique type of IBV.
1997年至1998年期间,从意大利和波兰采集了18株传染性支气管炎病毒(IBV)分离株,通过血清血凝抑制试验、病毒中和试验、型特异性聚合酶链反应以及刺突蛋白S1基因测序进行了全面分析。在意大利检测到了四种类型的IBV(793/B、624/I、B1648和马萨诸塞型),而在波兰证实存在793/B型。这表明,不仅在同一年中有四种类型的IBV共存,而且几种类型的IBV已在欧洲持续存在多年(B1648型和793/B型至少已存在13至14年)。对624/I分离株的S1基因进行测序,证实其为一种独特类型的IBV。