Kato M, Miura Y, Kettoku M, Shindo K, Iwamatsu A, Kobayashi K
a Applied Bioresearch Center , Kirin Brewery Co., Ltd. , 3 Mivaharacho, Takasakishi, Gunma 370-12 , Japan.
b Pharmaceutical Research Laboratories , Kirin Brewery Co., Ltd ., 3 Mivaharacho, Takasakishi, Gunma 370-12 , Japan.
Biosci Biotechnol Biochem. 1996 Jan;60(5):921-4. doi: 10.1271/bbb.60.921.
An amylolytic activity, which converts soluble starch to α,α-trehalose (trehalose), was found in the cell homogenate of the hyperthermophilic, acidophilic archaeum Sulfolobus solfataricus KM1. Two enzymes, a glycosyltransferase and an amylase, which are essential for this activity, were purified to homogeneity. A glycosyltransferase catalyzed the conversion of maltooligosaccharides to glycosyltrehaloses. Based on a detailed analysis of the reaction products, kinetic parameters, and an experiment using (3)H-labeled substrates, it was verified that glycosyltransferase transferred an oligomer segment of maltooligosaccharide to the Cl-OH position of glucose, located at the reducing end of the maltooligosaccharide, to produce a glycosyltrehalose having an α-1,1 linkage. The reaction appears to be intramolecular. Nine strains of the Sulfolobaceae family were found to have glycosyltransferases.
在嗜热嗜酸古菌嗜热栖热菌KM1的细胞匀浆中发现了一种淀粉分解活性，该活性可将可溶性淀粉转化为α,α-海藻糖（海藻糖）。对该活性至关重要的两种酶，一种糖基转移酶和一种淀粉酶，被纯化至同质。一种糖基转移酶催化麦芽寡糖转化为糖基海藻糖。基于对反应产物、动力学参数的详细分析以及使用(3)H标记底物的实验，证实糖基转移酶将麦芽寡糖的寡聚体片段转移至位于麦芽寡糖还原端的葡萄糖的Cl-OH位置，以产生具有α-1,1键的糖基海藻糖。该反应似乎是分子内反应。发现硫化叶菌科的九个菌株具有糖基转移酶。
