Gröndahl-Yli-Hannuksela Kirsi, Vahlberg Tero, Ilonen Jorma, Mertsola Jussi, He Qiushui
Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland.
Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland.
Immunogenetics. 2016 Oct;68(9):733-41. doi: 10.1007/s00251-016-0923-0. Epub 2016 Jun 10.
Individual variation in immune responses is always encountered after vaccination. This phenomenon is also seen after acellular pertussis vaccination. The aim of this present study was to investigate whether single nucleotide polymorphisms (SNPs) in the IL-10 gene promoter region (rs1800890, rs1800896, rs1800871), IL-12B (rs2546890), IL-12RB1 (rs372889), IL-17A (rs2275913), and IL-23R (rs11209026) affect the immune responses after acellular pertussis vaccination. The T cell proliferative response was evaluated in 38 Finnish young adults who received a second booster dose of a vaccine combination of diphtheria, tetanus, and acellular pertussis, 10 years after the previous booster. The response was evaluated with a proliferation assay in which vaccine antigens pertussis toxin (PT), filamentous hemagglutinin (FHA), and pertactin (PRN) were used for the stimulation, before and 1 month after the second vaccination. Specific proliferation of peripheral blood mononuclear cells against pertussis antigens was affected by IL-10 SNP in the promoter region at position -1082 (A>G, rs1800896). One month after the vaccination, subjects with the AA and AG genotypes had a significantly higher T cell proliferative response against PT and FHA compared to those with the GG genotype. Subjects with the GG genotype had the lowest responses. As a conclusion, our preliminary results indicate that IL-10 SNP -1082 might play an important role in T cell-mediated immune responses after acellular pertussis vaccination.
接种疫苗后总会出现免疫反应的个体差异。这种现象在无细胞百日咳疫苗接种后也有出现。本研究的目的是调查白细胞介素-10（IL-10）基因启动子区域（rs1800890、rs1800896、rs1800871）、白细胞介素-12B（IL-12B，rs2546890）、白细胞介素-12受体β1（IL-12RB1，rs372889）、白细胞介素-17A（IL-17A，rs2275913）和白细胞介素-23受体（IL-23R，rs11209026）中的单核苷酸多态性（SNP）是否会影响无细胞百日咳疫苗接种后的免疫反应。对38名芬兰年轻成年人的T细胞增殖反应进行了评估，这些人在之前一次加强免疫10年后接种了白喉、破伤风和无细胞百日咳疫苗组合的第二剂加强针。在第二次接种前和接种后1个月，通过增殖试验评估反应，其中使用百日咳毒素（PT）、丝状血凝素（FHA）和百日咳杆菌黏附素（PRN）等疫苗抗原进行刺激。外周血单个核细胞针对百日咳抗原的特异性增殖受到启动子区域-1082位IL-10 SNP（A>G，rs1800896）的影响。接种疫苗1个月后，与GG基因型的受试者相比，AA和AG基因型的受试者对PT和FHA的T细胞增殖反应显著更高。GG基因型的受试者反应最低。总之，我们的初步结果表明，IL-10 SNP -1082可能在无细胞百日咳疫苗接种后的T细胞介导的免疫反应中起重要作用。
