欧洲食品官方控制：采用高效液相色谱-紫外-二极管阵列检测法测定鱼制品中的组胺

European official control of food: Determination of histamine in fish products by a HPLC-UV-DAD method.

作者信息

Altieri I, Semeraro A, Scalise F, Calderari I, Stacchini P

机构信息

Veterinary Public Health and Food Safety Department, Food Chemical Safety and Human Health Unit, Istituto Superiore di Sanità, Rome, Italy.

出版信息

Food Chem. 2016 Nov 15;211:694-9. doi: 10.1016/j.foodchem.2016.05.111. Epub 2016 May 19.

DOI:10.1016/j.foodchem.2016.05.111

PMID:27283685

Abstract

The evaluation of histamine content in fish and fishery products, responsible for scombroid poisoning, is essential to guarantee the safety of food. EU regulation requires validated analytical methods to ensure the verification of compliance with food law in official control activity. To this aim a previous gradient RP-HPLC method with DAD detection was modified and validated, according to international guidelines. The reliability of results was tested by analysing fish reference materials within the participation in European proficiency tests. The method has been used for the analysis of real samples consisting of several fish-based products with considerable differences in matrix composition. This characteristic is of great relevance to be able of apply the method in the field of official control.

摘要

评估导致鲭鱼中毒的鱼类及渔业产品中的组胺含量，对于保证食品安全至关重要。欧盟法规要求采用经过验证的分析方法，以确保在官方控制活动中核查是否符合食品法。为此，根据国际指南，对先前采用二极管阵列检测的梯度反相高效液相色谱法进行了改进和验证。通过在参与欧洲能力验证试验期间分析鱼类标准物质，检验了结果的可靠性。该方法已用于分析由几种基质组成差异很大的鱼类制品构成的实际样品。这一特性对于能够在官方控制领域应用该方法具有重要意义。