Mössner J, Regner U F, Zeeh J M, Bruch H P, Eberlein G
Medizinische Poliklinik, University of Würzburg.
Z Gastroenterol. 1989 Feb;27(2):94-8.
Cholecystokinin (CCK) is a polypeptid released postprandially by the upper intestinal mucosa. There are several biological active forms of CCK. Radioimmunological measurements of CCK may not detect all biological active forms or may have the disadvantage of crossreacting with gastrin. In the following we describe a modification of a bioassay for CCK which was first developed by Liddle et al. (J Clin Invest 1985). By means of this bioassay pre- and postprandial plasma CCK-levels of healthy male volunteers are compared with CCK-levels of patients with partial gastric resections and excluded duodenum. Both groups showed similar basal CCK-values (about 1 pM) and a food induced increase of this hormone by reaching maximal values after 15 to 30 min (control: 4.30 +/- 0.65 vs. operated: 13.37 +/- 2.83 pM). Patients with gastric resections, however, had about three times more CCK released over the 60 min time period studied as compared to controls. Thus exclusion of the duodenum, the supposed main place of CCK production, does not cause a lower but rather higher increase of postprandial CCK release.
胆囊收缩素（CCK）是一种在餐后由上消化道黏膜释放的多肽。CCK有几种生物活性形式。CCK的放射免疫测定可能无法检测到所有生物活性形式，或者可能存在与胃泌素发生交叉反应的缺点。在下面的内容中，我们描述了一种对CCK生物测定法的改进，该方法最初由利德尔等人开发（《临床研究杂志》，1985年）。通过这种生物测定法，将健康男性志愿者餐后前后的血浆CCK水平与部分胃切除且十二指肠已切除患者的CCK水平进行比较。两组的基础CCK值相似（约1皮摩尔），进食后该激素水平升高，在15至30分钟后达到最大值（对照组：4.30±0.65皮摩尔，手术组：13.37±2.83皮摩尔）。然而，在研究的60分钟时间段内，胃切除患者释放的CCK比对照组多约三倍。因此，排除CCK假定的主要产生部位十二指肠，并不会导致餐后CCK释放量降低，反而会使其升高。
